Cumulative live birth rates in ICSI cycles with donated oocytes are not improved by magnetic-activated cell sorting: A retrospective study in unselected males

2021 ◽  
Author(s):  
M. Gil Juliá ◽  
I. Hervás ◽  
A. Navarro-Gomezlechon ◽  
F. Quintana ◽  
D. Amorós ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Live Birth ◽  
Cell Sorting ◽  
Birth Rates ◽  
Live Birth Rates ◽  
Cumulative Live Birth ◽  
Magnetic Activated Cell Sorting

scholarly journals Estradiol on Trigger Day was Irrelevant to Live Birth Rates of Fresh Cycles but Positively Associated with Cumulative Live Birth Rates: A Multicenter Retrospective Study

2021 ◽  
Author(s):  
Meng Wang ◽  
Lei Jin ◽  
Juanzi Shi ◽  
Yundong Mao ◽  
Cuilian Zhang ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Live Birth ◽  
Multilevel Models ◽  
Preimplantation Embryos ◽  
Birth Rates ◽  
Live Birth Rates ◽  
Cumulative Live Birth ◽  
Stage Embryo ◽  
Specific Stage

Abstract Background: Serum estradiol (E2) level is supraphysiological during controlled ovarian stimulation (COS). However, the impacts of high levels of E2 on the development of preimplantation embryos, live birth rates in fresh and frozen-thawed embryo transfer (FET) cycles, and cumulative live birth rates (CLBR) remain debatable.Methods: This retrospective study included 93,959 oocyte retrieval cycles of 73,794 patients performing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) from 2013 to 2018 in five reproductive centers in China. Patients were categorized according to E2 level on trigger day: <1,000, 1,000-2,000, 2,000-3,000, 3,000-4,000, 4,000-5,000 and ≥5,000 pg/ml. Developments of embryos were evaluated by specific stage embryo rates, fertilization rates and cleavage rates. Generalized linear mixed models and multilevel models were adopted. Univariate and multivariate analysis were performed to adjust for the confounding factors. Results: Compared with the <1,000 pg/ml group, the aMD (95% CI) for the specific stage embryo rates in groups 2-6 were -0.10 (-0.11, -0.10), -0.13 (-0.14, -0.12), -0.14 (-0.15, -0.13), -0.15 (-0.16, -0.15) and -0.14 (-0.15, -0.13), respectively. The adjusted odds ratio (aOR) and 95% confidence interval (CI) of live birth of fresh cycles in the stratified higher E2 groups was 1.03 (0.91, 1.16), 1.06 (0.93, 1.21), 1.03 (0.89, 1.19), 1.02 (0.87, 1.20) and 0.88 (0.75, 1.03), while the aOR (95% CI) for live birth in FET cycles was 1.01 (0.90, 1.14), 1.08 (0.95, 1.22), 1.10 (0.96, 1.27), 1.12 (0.97, 1.29), and 1.20 (1.05, 1.37), respectively. When E2 <14,000 pg/ml, E2 was positively correlated with CLBR, otherwise, E2 was irrelevant to CLBR. Conclusion: High levels of E2 is associated with decreased rates of properly developed embryos. When E2 level is above 5,000 pg/ml on trigger day, the E2 level is associated with increased live birth rates in FET cycles. While the live birth rates in fresh cycles is not impacted. CLBR is positively associated with E2 on trigger day when E2 is <14,000 pg/ml.

Cumulative live birth rates in in-vitro fertilization

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Panagiotis Drakopoulos ◽  
Joaquín Errázuriz ◽  
Samuel Santos-Ribeiro ◽  
Herman Tournaye ◽  
Alberto Vaiarelli ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Live Birth ◽  
Birth Rates ◽  
Vitro Fertilization ◽  
Live Birth Rates ◽  
Cumulative Live Birth

scholarly journals Sperm Selection by Magnetic-Activated Cell Sorting Before Microinjection of Autologous Oocytes Increases Cumulative Live Birth Rates with Limited Clinical Impact: A Retrospective Study in Unselected Males

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 430
Author(s):  
María Gil Juliá ◽  
Irene Hervás ◽  
Ana Navarro-Gómez Lechón ◽  
Fernando Quintana ◽  
David Amorós ◽  
...  
Keyword(s):  
Live Birth ◽  
Clinical Impact ◽  
Sperm Selection ◽  
Reproductive Outcomes ◽  
Birth Rates ◽  
Multicenter Observational Study ◽  
Live Birth Rates ◽  
Cumulative Live Birth ◽  
The Impact ◽  
Autologous Oocytes

The application of MACS non-apoptotic sperm selection in infertility clinics is controversial since the published literature does not agree on its effect on reproductive outcomes. Therefore, it is not part of the routine clinical practice. Classical measures of reproductive success (pregnancy or live birth rates per ovarian stimulation) introduce a bias in the evaluation of a technique’s effect, since only the best embryo is transferred. This retrospective, multicenter, observational study evaluated the impact of MACS on reproductive outcomes, measuring results in classical parameters and cumulative live birth rates (CLBR). Data from ICSI cycles using autologous oocyte in Spanish IVIRMA fertility clinics from January 2008 to February 2020 were divided into two groups according to their semen processing: standard practice (reference: 46,807 patients) versus an added MACS sperm selection (1779 patients). Only when measured as CLBR per embryo transferred and per MII oocyte used was the difference between groups statistically significant. There were no significant differences between MACS and reference groups on pregnancy and live birth rates. In conclusion, results suggest that non-apoptotic sperm selection by MACS on unselected males prior to ICSI with autologous oocytes has limited clinical impact, showing a subtle increase in CLBR per embryo transferred.

O-014 How effective is oocyte cryopreservation?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Cobo
Keyword(s):  
Cancer Patients ◽  
Live Birth ◽  
Ovarian Reserve ◽  
Oocyte Retrieval ◽  
Success Rates ◽  
Birth Rates ◽  
Oocyte Vitrification ◽  
Live Birth Rates ◽  
Cumulative Live Birth ◽  
Different Populations

Abstract text The challenge of cryopreserve, store for prolonged period, and successfully implant the female gamete is nowadays feasible thanks to vitrification. The technology that was initially validated in oocyte recipients is currently applied to a vast population, including women at risk of losing their ovarian function due either to iatrogenic causes as occurs in cancer patients, or due to the natural depletion of the ovarian reserve as a result of age related fertility decline. That is the case of a growing population of women who wish to postpone childbearing and decide on oocyte vitrification as a means of fertility preservation (FP). At present, there is a growing body of evidence regarding the use of vitrified oocytes by many women under different indications, which makes it possible to evaluate the approach from different scenarios. So that vitrification can be evaluated in terms on survival rates, embryo development and the rate at which vitrified oocytes develop into live-born children in IVF cycles using vitrified oocytes which were initially stored due to different reasons. The effects of vitrification at the subcellular level and its impact on oocyte competence is of interest in the evaluation of the efficacy of the technology. Some studies have indicated that vitrification may affect ultrastructure, reactive oxygen species (ROS) generation, gene expression, and epigenetic status. However, it is still controversial whether oocyte vitrification could induce DNA damage in the oocytes and the resulting early embryos. Recent studies show that oocytes survival and clinical outcome after vitrification can be impaired by patients’ age and the clinical indication or the reason for vitrification. These studies show that age at oocyte retrieval strongly affects the survival and reproductive prognosis. In our experience, oocyte survival, pregnancy and cumulative live birth rates are significantly higher when patients are aged 35 years or younger versus patients older than 35 years at oocyte retrieval. Therefore, elective-FP patients should be encouraged to decide at young ages to significantly increase their chances of success. There is also evidence that the reason for vitrification is associated to the success rates. Poorer reproductive outcome was reported in cancer patients, low responders and endometriosis patients when compared to healthy women in age matching groups. Moreover, there are certain individualities linked to specific populations, as occurs when endometriosis patients had cystectomy earlier than the oocyte retrieval for FP. These women achieved lower success rates as compared to non-operated age matching counterparts. In this case, the lower cumulative live birth rates observed in operated women are, most probably, due to the smaller number of oocytes available, as a consequence of the detrimental effect of the surgery on the ovarian reserve. In this regard, several reports show that the number of oocytes available per patient is another variable closely related to the outcome in all populations using vitrified oocytes after FP. Thus, a significant improvement in the cumulative live birth rates can be achieved by adding a few oocytes, especially in healthy young patients. Different populations using vitrified oocytes under several indications achieve differential results in terms of pregnancy rates, when calculated in overall. Nonetheless, when the calculations for the cumulative probability of achieving a baby are made according the number of oocytes used per patient belonging to the same group of age, the results become comparable between different populations, as shown by the comparison between elective freezers versus endometriosis patients. Undoubtedly, vitrification can be recognized as one of the latest brakethrough in the ART field, but certainly the next step forward would be the successfull automatization of the vitrification and warming processes to achieve fully consistency among different laboratories.

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