Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology

2019 ◽  
Vol 141 ◽  
pp. 847-857 ◽  
Author(s):  
Mansour Shahedi ◽  
Maryam Yousefi ◽  
Zohreh Habibi ◽  
Mehdi Mohammadi ◽  
Mohammad Ali As'habi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Palm Oil ◽  
Rhizomucor Miehei ◽  
Candida Antarctica ◽  
Biodiesel Production ◽  
Candida Antarctica Lipase B ◽  
Rhizomucor Miehei Lipase ◽  
Candida Antarctica Lipase ◽  
Miehei Lipase

scholarly journals Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 831 ◽  
Author(s):  
Juan Calero ◽  
Diego Luna ◽  
Carlos Luna ◽  
Felipa Bautista ◽  
Beatriz Hurtado ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Silica Gel ◽  
Rhizomucor Miehei ◽  
Rhizomucor Miehei Lipase ◽  
Reaction Parameters ◽  
Experimental Conditions ◽  
Inorganic Solids ◽  
Lipozyme Rm Im ◽  
Miehei Lipase

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.

scholarly journals Kinetic Modeling and Optimization of Immobilized Candida antarctica Lipase B Catalysed Synthesis of Butyl-4-Methyl-3-Oxopentanoate using Response Surface Methodology

2012 ◽  
Vol 10 (1) ◽  
Author(s):  
Ganapati D. Yadav ◽  
Somnath Dattatray Shinde
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Polynomial Regression ◽  
Enzymatic Reaction ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Suitable Model ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Immobilized Candida Antarctica Lipase

Abstract Response surface methodology (RSM) was used to model and optimize the immobilized Candida antarctica lipase B catalysed synthesis of butyl-4-methyl-3-oxopentanoate. To determine optimum conditions of the transesterification, a four-factor and five-level central composite rotatable design (CCRD) was used. The factors studied were enzyme load (A), reaction temperature (B), methyl-4-methyl-3-oxopentanoate concentration (C) and n-butanol concentration (D). A quadratic polynomial regression model was used to analyze the experimental data at a 95% confidence level (p < 0.05). The results indicated that the RSM approach gave reasonable results for the optimization of the reaction parameters in the range of tested parameters. The optimal conditions for the enzymatic reaction were obtained at 0.01 mol of methyl-4-methyl-3-oxopentanoate and 0.03 mol of n-butanol using 104 mg of Novozym 435 at 55 °C and 300 rpm for 6 h. Under these conditions, the transesterification percentage was 87 %. Further, kinetic modelling of the enzymatic synthesis was illustrated. Initial rate data and progress curve data were used to arrive at a suitable model. The kinetics was found to obey the ternary complex ordered bi-bi model with inhibition by the substrate methyl-4-methyl-3-oxopentanoate. The values of kinetic parameters obtained from nonlinear regression analysis were found to be Vmax of 0.04 mol/L.min; Km(A) 0.11 mol/L; Km(B) 2 mol/L and Ki(A) 2.2 mol/L.

Parametric optimization of polycaprolactone synthesis catalysed by Candida antarctica lipase B using response surface methodology

Biopolymers ◽  
2018 ◽  
Vol 109 (12) ◽  
pp. e23240 ◽  
Author(s):  
Harshini Pakalapati ◽  
Senthil Kumar Arumugasamy ◽  
Jegalakshimi Jewaratnam ◽  
Yong Jie Wong ◽  
Mohammad Khalid
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Parametric Optimization ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B

Rapid and high-density covalent immobilization of Rhizomucor miehei lipase using a multi component reaction: application in biodiesel production

RSC Advances ◽  
2015 ◽  
Vol 5 (41) ◽  
pp. 32698-32705 ◽  
Author(s):  
Mehdi Mohammadi ◽  
Maryam Ashjari ◽  
Shaghayegh Dezvarei ◽  
Maryam Yousefi ◽  
Mohadese Babaki ◽  
...  
Keyword(s):  
Mild Condition ◽  
High Capacity ◽  
Rhizomucor Miehei ◽  
Covalent Immobilization ◽  
Ugi Reaction ◽  
High Density ◽  
Biodiesel Production ◽  
Rhizomucor Miehei Lipase ◽  
Miehei Lipase

Rapid and high capacity immobilization of Rhizomucor miehei lipase on aldehyde-functionalized supports was performed under mild condition via a multi component reaction. The mechanism of immobilization reaction was determined as the Ugi reaction.

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