Characterization of Borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after MluI restriction of genomic DNA

The aim of this study was to characterize genetically Bulgarian Erwinia amylovora strains using pulsed-field gel electrophoresis (PFGE) analysis. Fifty E. amylovora strains isolated from different hosts, locations, as well as in different years were analysed by PFGE after XbaI, SpeI, and XhoI digestion of the genomic DNA. The strains were distributed into four groups according to their XbaI-generated profile. About 82% of the strains displayed a PFGE profile identical to that of type Pt2. Three strains belonged to the Central Europe Pt1 type. Two new PFGE profi les, not reported so far, were established - one for a strain isolated from Malus domestica and another for all Fragaria spp. strains. The same grouping of the strains was obtained after analysis of the SpeI digestion patterns. On the basis of PFGE profiles, after XbaI and SpeI digestion, a genetic differentiation between the strains associated with subfamily Maloideae and subfamily Rosoideae was revealed. The presence of more than one PFGE profi le in the population of E. amylovora in Bulgaria suggests a multiple source of inoculum

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Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis.

Journal of Clinical Microbiology ◽

10.1128/jcm.31.11.2873-2877.1993 ◽

1993 ◽

Vol 31 (11) ◽

pp. 2873-2877 ◽

Cited By ~ 50

Author(s):

J Belfaiza ◽

D Postic ◽

E Bellenger ◽

G Baranton ◽

I S Girons

Keyword(s):

Borrelia Burgdorferi ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Borrelia Burgdorferi Sensu Lato ◽

Genomic Fingerprinting ◽

Pulsed Field

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Characterization of Staphylococcus intermedius from Pigeons, Dogs, Foxes, Mink, and Horses by Pulsed-Field Gel Electrophoresis

Journal of Veterinary Medical Science ◽

10.1292/jvms.64.237 ◽

2002 ◽

Vol 64 (3) ◽

pp. 237-243 ◽

Cited By ~ 11

Author(s):

Yoshihisa WAKITA ◽

Akira SHIMIZU ◽

Václav HÁJEK ◽

Junichi KAWANO ◽

Kenji YAMASHITA

Keyword(s):

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Pulsed Field ◽

Staphylococcus Intermedius

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Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

Epidemiology and Infection ◽

10.1017/s0950268800051487 ◽

1994 ◽

Vol 113 (1) ◽

pp. 75-81 ◽

Cited By ~ 71

Author(s):

F. M. Khambaty ◽

R. W. Bennett ◽

D. B. Shah

Keyword(s):

Gel Electrophoresis ◽

Pfge Pattern ◽

Pulsed Field Gel Electrophoresis ◽

Western United States ◽

Single Strain ◽

Pulsed Field ◽

Definitive Evidence ◽

Staphylococcus Intermedius ◽

Epidemiological Characterization

SUMMARYAn outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991.Staphylococcus intermediuswas implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of sevenS. intermediusstrains of veterinary origin and five unrelatedS. aureuslaboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of differentStaphylococcusspecies but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization ofS. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused byS. intermedius. These findings suggest that the presence ofS. intermediusand other species such asS. hyicusin food should be reason for concern.

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