scholarly journals Repeated exposure of jacket plum (Pappea capensis) micro-cuttings to indole-3-butyric acid (IBA) improved in vitro rooting capacity

2007 ◽  
Vol 73 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Simon A. Mng'omba ◽  
Elsa S. Du Toit ◽  
Festus K. Akinnifesi ◽  
Helena M. Venter
HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514e-514
Author(s):  
Barbara M. Reed

Cultures of 49 Pyrus species and cultivars and one Pyronia (Pyrus × Cydonia hybrid) selection were screened in vitro to determine a rooting method suitable for a wide range of germplasm. Auxin treatment was required for rooting in most cases. Eighteen of the 50 accessions rooted with a 15 sec. 10 mM indole-3-butyric acid (IBA) dip followed by growth on medium with no growth regulators (NCR). Medium with 10 μM IBA for one week followed by NCR medium produced 12 rooted accessions, but NCR medium alone produced little or no rooting. A 15 sec. dip in 10 mM naphthaleneacetic acid (NAA) followed by NCR medium was tested on 29 accessions which rooted poorly on the other three treatments. Twice as many (28%) rooted on NAA as on either IBA treatment (14% each). Additional treatments combining IBA with darkness or higher temperature were also tested and were successful for some cultivars. P. calleryana, P. koehnei, P. pashia, P. hondoensis, P. ussuriensis, P. betulifolia, P. regelii, P. pyrifolia hybrid cv. Shinseiki and the Pyronia selection failed to root. Twenty two of the 32 P. communis cultivars rooted on at least one treatment.


HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1650-1651 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson

Hypocotyl cuttings were prepared from Ii-week-old aseptically grown seedlings of Fraser fir [Abies fraseri (Pursh) Poir.] and cultured 18 days on media containing 0 to 40 mg IBA/liter followed by transfer to the same medium without auxin. Greatest rooting (66%) occurred after treatment with 20 mg IBA/liter, whereas the greatest number of roots per rooted cutting (7.4) was noted following treatment with 40 mg·liter-1. Chemical name used: 1H-indole-3-butyric acid (IBA).


2004 ◽  
Vol 3 (2) ◽  
pp. 196-199 ◽  
Author(s):  
M.M.H. Molla ◽  
M. Dilafroza Khanam . ◽  
M.M. Khatun . ◽  
M. Al-Amin . ◽  
M.A. Malek .

2001 ◽  
pp. 53-59
Author(s):  
Katalin Magyarné Tábori ◽  
Judit Dobránszki ◽  
Antal Ferenczy ◽  
Erzsébet Jámborné Benczúr ◽  
János Lazányi

Effects of media hormone content on in vitro shoot multiplication and rooting were examined in cv. Red Fuji and McIntosh apple scions. Multiplication responses of shoots to different concentrations (0.5 and 1.0 mg/l) of 6-benzylaminopurine and 6-benzylaminopurine riboside were tested at two levels (0.1 and 0.3 mg/l) of indole-3-butyric acid. The best proliferation rate was achieved on medium containing 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l indole-3-butyric acid in cv. Red Fuji (5.3) and on medium containing 1.0 mg/l 6-benzylaminopurine and 0.3 mg/lindole-3-butyric acid in cv McIntosh (10.3). The length of shoots on these media was enough for rooting (38.4 mm in cv. Red Fuji and 39.3 mm in cv McIntosh). Shoots developed on the best proliferation medium were used for rooting. Effects of different concentrations of auxin in the root induction media and presence of activated charcoal in the root elongation media were examined on rooting capacity. The best rooting rate (100% in cv. McIntosh and 83% in cv. Red Fuji) was achieved when the root induction medium contained 1.0 mg/l indole-3-butyric acid. In general, rooting was inhibited in the presence of activated charcoal.  Because of high in vitro multiplication and rooting rate and high percent of survival during acclimatisation, the methods described here make effective micropropagation processes possible for cv. Red Fuji and McIntosh. 


HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1304-1305 ◽  
Author(s):  
James R. Ault

Shoot formation was obtained from Lachenalia arbuthnotiae W.F. Barker, L. bulbifera (Cyrillo) Engl., and L. purpureo-coerulea Jacq. leaf tissue explants cultured on Murashige and Skoog (MS) medium supplemented with sucrose at 30 g·liter–1, 8.87 μm BA, and 0.44 μm K-NAA. Shoots of all three species rooted on subculture to MS medium supplemented with 0.0, 4.14, or 8.29 μm K-IBA or 0.0, 4.46, or 8.92 μm K-NAA. Maximum percent rooting was ≈81% from treatment with 4.14 μm K-IBA for L. arbuthnotiae and with 8.29 μm K-IBA for L. purpureo-coerulea; it was 59% from treatment with 8.92 μm K-NAA for L. bulbifera. Rooted and nonrooted shoots were acclimatized in a greenhouse. Survival of rooted plants was 93% for L. arbuthnotiae, 95% for L. bulbifera, and 94% for L. purpureo-coerulea. Survival of nonrooted shoots was 71% for L. arbuthnotiae and 91% for L. bulbifera. Chemical names used: 6-benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthaleneacetic acid (K-NAA).


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