scholarly journals In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1304-1305 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Butyric Acid ◽  
Potassium Salt ◽  
Leaf Tissue ◽  
Shoot Formation ◽  
In Vitro Rooting ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Tissue Explants

Shoot formation was obtained from Lachenalia arbuthnotiae W.F. Barker, L. bulbifera (Cyrillo) Engl., and L. purpureo-coerulea Jacq. leaf tissue explants cultured on Murashige and Skoog (MS) medium supplemented with sucrose at 30 g·liter–1, 8.87 μm BA, and 0.44 μm K-NAA. Shoots of all three species rooted on subculture to MS medium supplemented with 0.0, 4.14, or 8.29 μm K-IBA or 0.0, 4.46, or 8.92 μm K-NAA. Maximum percent rooting was ≈81% from treatment with 4.14 μm K-IBA for L. arbuthnotiae and with 8.29 μm K-IBA for L. purpureo-coerulea; it was 59% from treatment with 8.92 μm K-NAA for L. bulbifera. Rooted and nonrooted shoots were acclimatized in a greenhouse. Survival of rooted plants was 93% for L. arbuthnotiae, 95% for L. bulbifera, and 94% for L. purpureo-coerulea. Survival of nonrooted shoots was 71% for L. arbuthnotiae and 91% for L. bulbifera. Chemical names used: 6-benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthaleneacetic acid (K-NAA).

scholarly journals 578 PB 059 IN VITRO ROOTING OF PYRUS GERMPLASM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514e-514
Author(s):  
Barbara M. Reed
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
In Vitro Rooting ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Auxin Treatment ◽  
Wide Range ◽  
Hybrid Selection ◽  
Higher Temperature

Cultures of 49 Pyrus species and cultivars and one Pyronia (Pyrus × Cydonia hybrid) selection were screened in vitro to determine a rooting method suitable for a wide range of germplasm. Auxin treatment was required for rooting in most cases. Eighteen of the 50 accessions rooted with a 15 sec. 10 mM indole-3-butyric acid (IBA) dip followed by growth on medium with no growth regulators (NCR). Medium with 10 μM IBA for one week followed by NCR medium produced 12 rooted accessions, but NCR medium alone produced little or no rooting. A 15 sec. dip in 10 mM naphthaleneacetic acid (NAA) followed by NCR medium was tested on 29 accessions which rooted poorly on the other three treatments. Twice as many (28%) rooted on NAA as on either IBA treatment (14% each). Additional treatments combining IBA with darkness or higher temperature were also tested and were successful for some cultivars. P. calleryana, P. koehnei, P. pashia, P. hondoensis, P. ussuriensis, P. betulifolia, P. regelii, P. pyrifolia hybrid cv. Shinseiki and the Pyronia selection failed to root. Twenty two of the 32 P. communis cultivars rooted on at least one treatment.

scholarly journals Micropropagation of the Rare Lakeside Daisy (Hymenoxys acaulis var. glabra)

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 200-201 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Potassium Salt ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Stem Segment ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation

Shoot tip and stem segment explants collected from greenhouse-maintained plants of Hymenoxys acaulis var. glabra were cultured in vitro for shoot initiation on a Murashige and Skoog (MS) medium supplemented with 30 g·L-1 sucrose, 2.5 μm BA, and 7 g·L-1 agar at a pH of 5.7. Unbranched shoot explants were subcultured to MS medium with 0.0, 0.5, 1, 2, 4 or 8 μm BA for shoot proliferation. A maximum of 10.3 shoots per explant was produced on the medium with 2.0 μm BA. Nonrooted shoots were subcultured to MS medium with 0.0, 0.5, 2, or 8 μm K-IBA for rooting. Maximum rooting was 90% on MS medium with 0.5 μm K-IBA. Rooted shoots were greenhouse-acclimatized for 10 days. Overall survival was 75%. Chemical names used: 6-benzyl adenine (BA); potassium salt of indole-3-butyric acid (K-IBA).

scholarly journals In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

2017 ◽  
Vol 15 (10) ◽  
pp. 701-710
Author(s):  
Piyaporn SAENSOUK ◽  
Surapon SAENSOUK ◽  
Phattaraporn PIMMUEN
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Root Formation ◽  
Survival Rates ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Different Types

An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

scholarly journals In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1132-1135 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Rosario Julieta Baltazar-García ◽  
Victor Manuel Chávez-Avila
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation

A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg·L−1) and/or α-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg·L−1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg·L−1 in combination with at NAA 0.1 mg·L−1. The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without plant growth regulators, but with 1 g·L−1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

Establishment of a highly efficient regeneration system for in vitro culture of young wheat spikes

2006 ◽  
Vol 3 (2) ◽  
pp. 147-154
Author(s):  
Zhao Lin-Shu ◽  
Liu Lu-Xiang ◽  
Wang Jing ◽  
Zheng Qi-Cheng ◽  
Guo Hui-Jun ◽  
...  
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Immature Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration System ◽  
Differentiation Medium ◽  
Efficient Regeneration ◽  
Young Spike

AbstractThis study used three winter wheat (Triticum aestivum L.) genotypes (H6756, H311 and SP8581) to compare the effects of sampling time, callus induction media, differentiation media and rooting media on in vitro culture of young spikes in wheat. In all these three genotypes, the frequencies of green plantlet differentiation were high when their young spikes were cultured between the stages of protective glume primordium formation and pistil and stamen primordium formation, but low at other stages. The optimum medium for callus induction was Murashige and Skoog (MS) medium+2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum green plantlet differentiation medium was MS medium. Some abnormal plantlets regenerated from calli. When these plantlets were transferred to another differentiation medium [MS+1.0 mg/l 1-naphthaleneacetic acid (NAA)+0.2 mg/l 6-benzylaminopurine (6-BA)], shoot formation and elongation were induced. This allowed 90.91% of them to develop into normal green plantlets. The optimum rooting medium was 1/2MS+0.2 mg/l 3-Indolylacetonitrile (IAA)+80 g/l sucrose. An efficient regeneration system for young spike culture of wheat was set up based on such methods. Using this wheat-regeneration system, young spikes and immature embryos of 17 genotypes of wheat were in vitro cultured to study and compare the callus induction frequencies and green plantlet differentiation frequencies. The results of two successive years showed that in 15 out of the 17 genotypes (88.24%) the green plantlet differentiation frequencies were higher than those of immature embryos by 6.2–65.1%. These results showed that the regeneration system established in this trial for young spike culture of wheat was effective.

scholarly journals Micropropagation of Paphiopedilum x Dalatense

2021 ◽  
Vol 19 (1) ◽  
pp. 155-163
Author(s):  
Tran Thai Vinh ◽  
H’ Yon Niê Bing ◽  
Dang Thi Tham ◽  
Nguyen Thi Thanh Hang ◽  
Vu Kim Cong ◽  
...  
Keyword(s):  
Survival Rate ◽  
Humic Acid ◽  
Shoot Formation ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Orchid Species ◽  
Best Response ◽  
Yeast Powder ◽  
Half Strength

Paphiopedilum x dalatense is a beautiful orchid species with large flowers in variable colors and leaves covered with stripes and beautiful unseen mosaic spots. Recently, many people exploit this species, causing it becomes very rare. In this study, we studied the effects of various organic matter: potato, banana and tryptone, yeast powder, peptone on the growth and development of P. dalatense shoots as well as the effects of NAA and humic acid on in vitro rooting of this orchid were investigated. The research results showed that MS medium supplemented with 100 g/L banana in combination with 100 g/L potato (5,4 shoots/sample, 18,8 mm/shoot, 4,5 leaves/shoot, and shoots survival rate of 100%) or MS medium supplemented with 1 g/L peptone (4,19 shoots/sample, 15 mm/shoot, 4 leaves/bud, and 92% of shoots survival rate) were the best response for the shoot formation and development. In addition, the half strength MS culture medium supplemented with 1 mg/L NAA (5,2 leaves/sample, 4,6 roots/buds, 3,56 cm/root, and 100% rate for rooting) was the suitable medium for the in vitro rooting of P. dalatense. Being cultured on half strength MS medium supplemented with 2 mg/L humic acid, the rooting rate reached 100% with the greatest root number and the longest root (5 roots/shoots, 5,5 cm/root). The obtained results on the in vitro propagation on this orchid helps contribute to the conservation and increases the genotic pool of this precious wild orchid species, as well as the rapid multiplication of healthy plantlets serving the commercialization of precious orchid species.

scholarly journals In Vitro Regeneration of Triploid Plants of Euonymus alatus ‘Compactus’ (Burning Bush) from Endosperm Tissues

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1141-1147 ◽  
Author(s):  
Chandra Thammina ◽  
Mingyang He ◽  
Litang Lu ◽  
Kaishuang Cao ◽  
Hao Yu ◽  
...  
Keyword(s):  
Butyric Acid ◽  
In Vitro Regeneration ◽  
The United States ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Euonymus Alatus ◽  
Non Invasive ◽  
Landscape Plant ◽  
Mature Endosperm

Euonymus alatus (Thunb.) Sieb., commonly known as “burning bush,” is an extremely popular landscape plant in the United States as a result of its brilliant showy red leaves in fall. However, E. alatus is also seriously invasive because of its prolific seed production and effective seed dispersal by birds. Thus, development of sterile, non-invasive, seedless triploid E. alatus is in high demand. In this article, we report successful production of triploid E. alatus using endosperm tissues as explants. In our study, ≈50% of immature endosperm explants and 14% of mature endosperm explants formed compact, green calli after culture in the dark for 8 weeks and then under light for 4 weeks on Murashige and Skoog (MS) medium supplemented with 2.2 μM BA and 2.7 μM α-naphthaleneacetic acid (NAA). Approximately 5.6% of the immature endosperm-derived calli and 13.4% of mature endosperm-derived calli initiated shoots within 8 weeks after they were cultured on MS medium with 4.4 μM benzyladenine (BA) and 0.5 μM indole-3-butyric acid (IBA). Eighty-five percent of shoots rooted after culture on woody plant medium (WPM) containing 4.9 μM IBA for 2 weeks and then on hormone-free WPM medium containing 2.0 g·L−1 activated charcoal for 4 weeks. Eight independently regenerated triploid plants have been identified. Triploid plant regeneration rates observed were 0.42% from immature endosperm explants and 0.34% from mature endosperm explants, respectively, based on the number of endosperm explants cultured. Because triploid plants cannot produce viable seeds, and thus are sterile and non-invasive, some triploid E. alatus plant lines reported here can be used to replace the currently used invasive counterparts. Chemical names used: benzyladenine (BA), indole-3-butyric acid (IBA), and α-naphthaleneacetic acid (NAA).

scholarly journals Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant

2012 ◽  
Vol 4 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Mohammad Serajur RAHMAN ◽  
Mohammad Abdul Bari MIAH ◽  
Mohammad Shahadat HOSSAIN ◽  
Ahmad Humayan KABIR ◽  
Mohammad Motiur RAHMAN
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Liquid Media ◽  
Indolebutyric Acid ◽  
Abrus Precatorius ◽  
Isolated Cell

A new protocol has been developed for cell culture and in vitro regeneration of Abrus precatorius that holds enormous potentiality for preparation of medicines. In vitro grown calli were cultured in Murashige and Skoog (MS) liquid media in agitated condition fortified with 0.5 mg/l 6-Benzylaminopurine. Growth curve of cells revealed that the cells continued to grow until 12 days of culture and got the highest peak from day 6-8. Isolated cell was found to produce highest 8.2% calli when suspended on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. Callus derived from single cell produced highest number of embryo (25-28%) cultured on MS medium fortified with 2.0 mg/l 6-Benzylaminopurine and 0.2 mg/l 1-Naphthaleneacetic acid. The bipolar embryos were selected and optimum shoot formation was recorded on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. The optimum root induction was noticed in MS medium supplemented with 1.0 mg/l 3-Indolebutyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

MICROPROPAGATION AND IN VITRO STORAGE OF CENTAURIUM RIGUALII ESTEVE (GENTIANACEAE)

1996 ◽  
Vol 44 (2-3) ◽  
pp. 115-123 ◽  
Author(s):  
José M. Iriondo ◽  
César Pérez
Keyword(s):  
Survival Rate ◽  
Somaclonal Variation ◽  
Plant Species ◽  
Butyric Acid ◽  
Naphthaleneacetic Acid ◽  
Endangered Plant ◽  
Ms Medium ◽  
Secondary Products ◽  
Chromosome Counts

Centaurium rigualii Esteve, an endangered plant species from southeastern Spain with potentially useful secondary products, was successfully micropropagated from shoot tips using MS supplemented with 0.44 μM 6- benzylaminopurine (BA) and 0.05 μM 1-naphthaleneacetic acid (NAA) at the proliferation step, and MS medium alone or with varying concentrations of indole-3-butyric acid (IBA) at the rooting step. No incidence of somaclonal variation was detected among randomly chosen shoots from ten subclonal lines in eight isozyme systems studied and in chromosome counts. However, this does not preclude the existence of somaclonal variation at other levels of the genome. A 90% survival rate was obtained when shoots were stored for three years in MS medium supplemented with 4.92 μM IBA at 5 °C and with a 16-h photoperiod.

scholarly journals Micropropagation and Polyploid Induction of Acer platanoides ‘Crimson Sentry

2013 ◽  
Vol 31 (4) ◽  
pp. 246-252 ◽  
Author(s):  
Jason D. Lattier ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Jeremy C. Smith
Keyword(s):  
In Vitro Rooting ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Norway Maple ◽  
Future Improvement ◽  
Half Strength ◽  
Improvement Programs ◽  
Indole 3 Acetic Acid

Protocols were developed for micropropagation and induction of autopolyploids in a fastigiate cultivar of Norway maple (A. platanoides L. ‘Crimson Sentry’). Murashige and Skoog (MS) medium, woody plant medium (WPM), and Quoirin and Lepoivre medium were supplemented with 2 μM 6-benzylaminopurine (BA), meta-Topolin, 6-(γ,γ-dimethylallylamino) purine, kinetin, or thidiazuron to evaluate microshoot proliferation. Murashige and Skoog medium with 2 μM BA yielded the most microshoots (3.2) and longest microshoots (30.6 mm) per subsample after 5 weeks. The influence of BA concentration on proliferation was evaluated at 0, 2, 4, 8, or 16 μM. Optimal multiplication rate was achieved at 2 or 4 μM BA producing approximately 2.8 microcuttings per subsample after 5 weeks. To induce in vitro rooting, half-strength WPM was supplemented with 0, 5, 10, 20, 40, or 80 μM indole-3-butyric acid (IBA). Optimal in vitro rooting (70%), number of roots (2.5), and root length (15 mm) per subsample were achieved with 10 μM IBA after 8 weeks. To induce polyploidy, microcuttings were pretreated for 7 days on MS medium with 4 μM BA alone or combined with 1 μM IBA, indole-3-acetic acid (IAA), or 1-naphthaleneacetic acid prior to treatment in liquid MS medium containing 15 μM oryzalin for 3 days. Homogenous tetraploids were only obtained from shoots pretreated with IAA. This research provides optimized protocols for micropropagation and autopolyploid induction of A. platanoides ‘Crimson Sentry’ and demonstrates the development of tetraploid lines for use in future improvement programs.

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