In vitro Rooting and Ex vitro Plantlet Establishment of BARI Banana 1 (Musa sp.) as Influenced by Different Concentration of IBA (Indole 3-butyric Acid)

2004 ◽  
Vol 3 (2) ◽  
pp. 196-199 ◽  
Author(s):  
M.M.H. Molla ◽  
M. Dilafroza Khanam . ◽  
M.M. Khatun . ◽  
M. Al-Amin . ◽  
M.A. Malek .
Keyword(s):  
Butyric Acid ◽  
In Vitro Rooting ◽  
Ex Vitro ◽  
Musa Sp

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Rooting of Vaccinium corymbosum L. microshoots cv. «Blue-Berry» in culture in vitro and ex vitro

2018 ◽  
Vol 7 (4) ◽  
pp. 80-84
Author(s):  
Gamil El-Dis Mohamed ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Survival Rate ◽  
Root System ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Vaccinium Corymbosum ◽  
Ex Vitro ◽  
Axillary Shoots ◽  
Maximum Increase ◽  
Regenerated Plantlets

This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. Blue-Berry. The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. Blue-Berry. The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,5-4) showed a 100% survival rate.

scholarly journals 578 PB 059 IN VITRO ROOTING OF PYRUS GERMPLASM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514e-514
Author(s):  
Barbara M. Reed
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
In Vitro Rooting ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Auxin Treatment ◽  
Wide Range ◽  
Hybrid Selection ◽  
Higher Temperature

Cultures of 49 Pyrus species and cultivars and one Pyronia (Pyrus × Cydonia hybrid) selection were screened in vitro to determine a rooting method suitable for a wide range of germplasm. Auxin treatment was required for rooting in most cases. Eighteen of the 50 accessions rooted with a 15 sec. 10 mM indole-3-butyric acid (IBA) dip followed by growth on medium with no growth regulators (NCR). Medium with 10 μM IBA for one week followed by NCR medium produced 12 rooted accessions, but NCR medium alone produced little or no rooting. A 15 sec. dip in 10 mM naphthaleneacetic acid (NAA) followed by NCR medium was tested on 29 accessions which rooted poorly on the other three treatments. Twice as many (28%) rooted on NAA as on either IBA treatment (14% each). Additional treatments combining IBA with darkness or higher temperature were also tested and were successful for some cultivars. P. calleryana, P. koehnei, P. pashia, P. hondoensis, P. ussuriensis, P. betulifolia, P. regelii, P. pyrifolia hybrid cv. Shinseiki and the Pyronia selection failed to root. Twenty two of the 32 P. communis cultivars rooted on at least one treatment.

scholarly journals A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

2020 ◽  
Vol 36 ◽  
pp. 66-74
Author(s):  
Mohammad Javad Mahmoudi Meimand ◽  
Mohammad Hossein Shamshiri ◽  
Khalil Malekzadeh ◽  
Mohammad Reza Dehghani
Keyword(s):  
Sucrose Concentration ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
The Media ◽  
Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efficiency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

scholarly journals Influence of propagation method a nd indole-3-butyric acid on growth and development of in vitro- and ex vitro-derived lingonberry plants

2006 ◽  
Vol 86 (1) ◽  
pp. 235-243 ◽  
Author(s):  
Samir C Debnath
Keyword(s):  
Butyric Acid ◽  
Growth And Development ◽  
Shoot Growth ◽  
Adventitious Shoot ◽  
Ex Vitro ◽  
Adventitious Shoot Regeneration ◽  
Propagation Methods ◽  
Rhizome Morphology ◽  
Rhizome Development

The effects of two propagation methods and four indole-3-butyric acid (IBA) concentrations were studied in two cultivars of lingonberry (Vaccinium vitis-idaea L.). Cultivars Regal and Erntedank, propagated by conventional softwood cuttings (SC) and adventitious shoot regeneration from excised leaves of micropropagated shoots (LC), were evaluated for growth and morphology. Significant two- and three-way interactions for shoot and rhizome characteristics were observed among the treatments. The LC plants produced shorter and less-vigorous shoots but had more stems, branches, leaves and rhizomes in contrast to conventional cuttings, which rarely produced rhizomes. IBA had an effect on shoot and rhizome morphology, but not on the frequency of rhizome formation. In vitro culture on zeatin-containing nutrient medium apparently induces the juvenile branching characteristics that favored enhanced rhizome production. The advantage of shoot and rhizome production of adventitiously produced LC plants over SC plants varied between the genotypes. Erntedank plants had better shoot growth and rhizome development across propagation methods than did Regal plants. Key words: Vaccinium vitis-idaea, propagation, regeneration, cuttings, indole-3-butyric acid

scholarly journals In Vitro Rooting of Hypocotyl Cuttings of Fraser Fir

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1650-1651 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
In Vitro Rooting ◽  
Rooted Cutting ◽  
Abies Fraseri ◽  
Fraser Fir ◽  
Hypocotyl Cuttings

Hypocotyl cuttings were prepared from Ii-week-old aseptically grown seedlings of Fraser fir [Abies fraseri (Pursh) Poir.] and cultured 18 days on media containing 0 to 40 mg IBA/liter followed by transfer to the same medium without auxin. Greatest rooting (66%) occurred after treatment with 20 mg IBA/liter, whereas the greatest number of roots per rooted cutting (7.4) was noted following treatment with 40 mg·liter-1. Chemical name used: 1H-indole-3-butyric acid (IBA).

scholarly journals Repeated exposure of jacket plum (Pappea capensis) micro-cuttings to indole-3-butyric acid (IBA) improved in vitro rooting capacity

2007 ◽  
Vol 73 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Simon A. Mng'omba ◽  
Elsa S. Du Toit ◽  
Festus K. Akinnifesi ◽  
Helena M. Venter
Keyword(s):  
Butyric Acid ◽  
Repeated Exposure ◽  
In Vitro Rooting ◽  
Rooting Capacity
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