A short-chain dehydrogenase/reductase (SDR) detection for the isoflavone reductase gene in Bulbophyllum and other orchids

2022 ◽  
Vol 144 ◽  
pp. 295-304
Author(s):  
Kisana Bhinija ◽  
Pattana Srifah Huehne ◽  
Skorn Mongkolsuk ◽  
Somkid Sitthimonchai ◽  
Jutamaad Satayavivad
Keyword(s):  
Short Chain ◽  
Reductase Gene ◽  
Isoflavone Reductase ◽  
Short Chain Dehydrogenase

scholarly journals Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9.

2007 ◽  
Vol 54 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Shen Liu ◽  
Chaoqun Huang ◽  
Dawei Li ◽  
Weihua Ren ◽  
Haoxing Zhang ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
Peptide Sequence ◽  
Short Chain ◽  
Signal Peptide Sequence ◽  
Liver Cdna Library ◽  
Reductase Gene ◽  
New Gene ◽  
Short Chain Dehydrogenase ◽  
Soluble Recombinant Protein

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

scholarly journals Medium- and short-chain dehydrogenase/reductase gene and protein families

2008 ◽  
Vol 65 (24) ◽  
pp. 3895-3906 ◽  
Author(s):  
K. L. Kavanagh ◽  
H. Jörnvall ◽  
B. Persson ◽  
U. Oppermann
Keyword(s):  
Short Chain ◽  
Protein Families ◽  
Reductase Gene ◽  
Short Chain Dehydrogenase

scholarly journals Medium- and short-chain dehydrogenase/reductase gene and protein families

2008 ◽  
Vol 65 (24) ◽  
pp. 3936-3949 ◽  
Author(s):  
X. Parés ◽  
J. Farrés ◽  
N. Kedishvili ◽  
G. Duester
Keyword(s):  
Short Chain ◽  
Protein Families ◽  
Reductase Gene ◽  
Short Chain Dehydrogenase
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