scholarly journals Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9.

2007 ◽  
Vol 54 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Shen Liu ◽  
Chaoqun Huang ◽  
Dawei Li ◽  
Weihua Ren ◽  
Haoxing Zhang ◽  
...  

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

2021 ◽  
Vol 348 ◽  
pp. 129046
Author(s):  
Mengyang Xing ◽  
Yong Chen ◽  
Boqiang Li ◽  
Shiping Tian

2022 ◽  
Vol 144 ◽  
pp. 295-304
Author(s):  
Kisana Bhinija ◽  
Pattana Srifah Huehne ◽  
Skorn Mongkolsuk ◽  
Somkid Sitthimonchai ◽  
Jutamaad Satayavivad

2017 ◽  
Vol 276 ◽  
pp. 88-94 ◽  
Author(s):  
Mark K. Adams ◽  
Seung-Ah Lee ◽  
Olga V. Belyaeva ◽  
Lizhi Wu ◽  
Natalia Y. Kedishvili

2009 ◽  
Vol 178 (1-3) ◽  
pp. 117-126 ◽  
Author(s):  
Yukuhiko Asada ◽  
Satoshi Endo ◽  
Yukari Inoue ◽  
Hiroaki Mamiya ◽  
Akira Hara ◽  
...  

FEBS Journal ◽  
2013 ◽  
Vol 280 (5) ◽  
pp. 1358-1370 ◽  
Author(s):  
Géraldine Buysschaert ◽  
Kenneth Verstraete ◽  
Savvas N. Savvides ◽  
Bjorn Vergauwen

1982 ◽  
Vol 218 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Jeffrey M. Davidson ◽  
Barbara Leslie ◽  
Terry Wolt ◽  
Ronald G. Crystal ◽  
Lawrence B. Sandberg

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