Cytotoxicity, genotoxicity and antimutagenicity of hexane extracts of Agaricus blazei determined in vitro by the comet assay and CHO/HGPRT gene mutation assay

A series of studies was performed to evaluate the safety of dihydrocapsiate (4-hydroxy-3-methoxybenzyl 8-methylnonanoate; CAS no. 205687-03-2). This study evaluated the potential genotoxicity of this compound using a variety of in vitro and in vivo test systems, including bacterial reverse mutation test, chromosomal aberration test, micronucleus test, gene mutation assay with transgenic rats, and single-cell gel (SCG) assay (Comet assay). In vitro tests (bacterial reverse mutation test and chromosomal aberration test) produced positive results in the absence of metabolic activation, but negative results in the presence of metabolic activation. The in vivo gene mutation assay (with transgenic rats) produced negative results, as did the in vivo mouse micronucleus assay, which failed to induce micronucleated polychromatic erythrocytes. Although the rat SCG assay produced statistically significant increases in the Olive tail moment and % tail DNA of the liver and intestine in the 2000 mg/kg group (compared with the negative-control group), a number of factors caused the authors to question the validity of these findings. Taken together, these results suggest that dihydrocapsiate has a low or extremely low likelihood of inducing genotoxicity.

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Evaluation of the genotoxic potential of some microbial volatile organic compounds (MVOC) with the comet assay, the micronucleus assay and the HPRT gene mutation assay

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/s1383-5718(01)00306-0 ◽

2002 ◽

Vol 513 (1-2) ◽

pp. 143-150 ◽

Cited By ~ 52

Author(s):

Ludwika Kreja ◽

Hans-Joachim Seidel

Keyword(s):

Volatile Organic Compounds ◽

Comet Assay ◽

Gene Mutation ◽

Organic Compounds ◽

Micronucleus Assay ◽

Hprt Gene ◽

Volatile Organic ◽

Microbial Volatile Organic Compounds ◽

Mutation Assay ◽

Gene Mutation Assay

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Comparison of in vitro micronucleus and gene mutation assay results for p53-competent versus p53-deficient human lymphoblastoid cells

Environmental and Molecular Mutagenesis ◽

10.1002/em.20634 ◽

2010 ◽

Vol 52 (5) ◽

pp. 373-384 ◽

Cited By ~ 24

Author(s):

Masamitsu Honma ◽

Makoto Hayashi

Keyword(s):

Gene Mutation ◽

Lymphoblastoid Cells ◽

Mutation Assay ◽

Gene Mutation Assay

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Toxicology of Diethylene Glycol Butyl Ether 3. Genotoxicity Evaluation in an In Vitro Gene Mutation Assay and an In Vivo Cytogenetic Test

Journal of the American College of Toxicology ◽

10.3109/10915819309140634 ◽

1993 ◽

Vol 12 (2) ◽

pp. 155-159 ◽

Cited By ~ 9

Author(s):

B. Bhaskar Gollapudi ◽

V. A. Linscombe ◽

M. L. Mcclintock ◽

A. K. Sinha ◽

C. R. Stack

Keyword(s):

Bone Marrow ◽

Gene Mutation ◽

Micronucleus Test ◽

Mouse Bone Marrow ◽

Butyl Ether ◽

Polychromatic Erythrocytes ◽

Mutation Assay ◽

Gene Mutation Assay

DGBE was evaluated in a forward gene mutation assay at the HGPRT locus of CHO cells in culture and in an in vivo mouse bone marrow micronucleus test for cytogenetic damage. DGBE did not elicit a positive response in the CHO/HGPRT assay when tested up to a maximum concentration of 5000 μg/ml with and without an external metabolic activation system (S-9). In the micronucleus test employing three post-treatment bone marrow sampling times (24, 48, and 72 hr), DGBE was ineffective in increasing the incidence of micronucleated polychromatic erythrocytes (MN-PCE) when tested in both sexes up to a maximum tolerated dose of 3300 mg/kg body weight. Thus, these data and those of others indicate a general lack of genotoxic potential for DGBE in short-term tests.

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