Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells

Author(s):  
Abdülkadir Şengün ◽  
Muhammet Yalçın ◽  
Hayriye Esra Ülker ◽  
Bora Öztürk ◽  
Sema S. Hakkı
2002 ◽  
Vol 28 (3) ◽  
pp. 188-192 ◽  
Author(s):  
G SCHMALZ ◽  
U SCHUSTER ◽  
A KOCH ◽  
H SCHWEIKL

2016 ◽  
Vol 8 (2) ◽  
pp. 63
Author(s):  
Salwani A. Ghani ◽  
Sam’an M. Masudi ◽  
Norshamsuria Omar

Dentin bonding agents are resin based materials that used in clinical dentistry inorder to prevent leakage and promote adherent of filling material to the enamel anddentin. However, the polymerization of dentin bonding agents will release residualmonomer that may interact with pulp tissue. This in vitro study is aimed to evaluatethe cytotoxicity of new 7thgeneration dentin bonding agents (G Bond) on stem cellsfrom human exfoliated deciduous teeth (SHEDs). The proliferated human pulp cellswere incubated at 37 C for 48 hours. Under aseptic conditions, extracted testspecimen were plated in 96 well of tissue tray. SHEDs were placed on each well withdifferent concentration of dentin bonding agents, and then incubated at 37 C for 72hours exposure. The cytotoxic effect was recorded by using MTT assay method.Statistical analysis showed that higher concentration of dentin bonding agents (IC=0.035 mg/ml)exerts higher toxic effect to the human pulp cells (SHEDs). This studyconcluded that pulpal reaction to dentin bonding agent may depend on number ofapplication procedure.


2003 ◽  
Vol 31 (3) ◽  
pp. 223-229 ◽  
Author(s):  
Ruey-Song Chen ◽  
Ching-Cheng Liu ◽  
Wan-Yu Tseng ◽  
Jiiang-Huei Jeng ◽  
Chun-Pin Lin

2003 ◽  
Vol 31 (7) ◽  
pp. 519
Author(s):  
Ruey-Song Chen ◽  
Ching-Cheng Liu ◽  
Wan-Yu Tseng ◽  
Jiiang-Huei Jeng ◽  
Chun-Pin Lin

2013 ◽  
Vol 29 ◽  
pp. e94
Author(s):  
A. Ionescu ◽  
E. Brambilla ◽  
M. Cadenaro ◽  
F. Tay ◽  
D. Pashley ◽  
...  

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