scholarly journals A cytotoxic evaluation of 7 th generation dentin bonding agent on human pulp cells

2016 ◽  
Vol 8 (2) ◽  
pp. 63
Author(s):  
Salwani A. Ghani ◽  
Sam’an M. Masudi ◽  
Norshamsuria Omar

Dentin bonding agents are resin based materials that used in clinical dentistry inorder to prevent leakage and promote adherent of filling material to the enamel anddentin. However, the polymerization of dentin bonding agents will release residualmonomer that may interact with pulp tissue. This in vitro study is aimed to evaluatethe cytotoxicity of new 7thgeneration dentin bonding agents (G Bond) on stem cellsfrom human exfoliated deciduous teeth (SHEDs). The proliferated human pulp cellswere incubated at 37 C for 48 hours. Under aseptic conditions, extracted testspecimen were plated in 96 well of tissue tray. SHEDs were placed on each well withdifferent concentration of dentin bonding agents, and then incubated at 37 C for 72hours exposure. The cytotoxic effect was recorded by using MTT assay method.Statistical analysis showed that higher concentration of dentin bonding agents (IC=0.035 mg/ml)exerts higher toxic effect to the human pulp cells (SHEDs). This studyconcluded that pulpal reaction to dentin bonding agent may depend on number ofapplication procedure.

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Robert Dalby ◽  
Ayman Ellakwa ◽  
Brian Millar ◽  
F. Elizabeth Martin

Objectives. To examine the effect of immediate dentin sealing (IDS), with dentin bonding agents (DBAs) applied to freshly cut dentin, on the shear bond strength of etched pressed ceramic luted to dentin with RelyX Unicem (RXU) cement.Method. Eighty extracted noncarious third molars were ground flat to expose the occlusal dentin surfaces. The teeth were randomly allocated to five groups (A to E) of sixteen teeth each. Groups A to D were allocated a dentin bonding agent (Optibond FL, One Coat Bond, Single Bond, or Go!) that was applied to the dentin surface to mimic the clinical procedure of IDS. These specimen groups then had etched glass ceramic discs (Authentic) luted to the sealed dentin surface using RXU. Group E (control) had etched glass ceramic discs luted to the dentin surface (without a dentin bonding agent) using RXU following the manufacturer’s instructions. All specimens were stored for one week in distilled water at room temperature and then shear stressed at a constant cross-head speed of 1 mm per minute until failure. Statistical analysis was performed by ANOVA followed by post hoc Tukey HSD method () applied for multiple paired comparisons.Results. The shear bond strength results for group A to E ranged from to  MPa. One-way ANOVA demonstrated a difference () between the groups tested and the Tukey HSD demonstrated a significant () difference between the shear bond strength (SBS) of Optibond FL (Group A) and Go! (Group D). There was no statistical difference () in the SBS between the test groups (A–D) or the control (group E).Conclusion. IDS using the dentin bonding agents tested does not statistically () affect the shear bond strength of etched pressed ceramic luted to dentin with RXU when compared to the control.


2012 ◽  
Vol 06 (02) ◽  
pp. 115-122 ◽  
Author(s):  
Bengi Oztas ◽  
Sebnem Kursun ◽  
Gul Dinc ◽  
Kıvanc Kamburoglu

ABSTRACTObjective: The purpose of this in vitro study was to explore the radiopacity of composite resins and bonding materials using film and phosphor plates.Methods: Nine composite dental resin specimens and human tooth slices were exposed together with an aluminium stepwedge using dental film and phosphor plates. Eight dentin bonding specimens were prepared and exposed in a similar manner. Their radiopacity on film was assessed using a transmission densitometer, and the radiopacity with phosphor plates was assessed digitally using the system’s own software (Digora). Data were analysed using one-way analysis of variance (ANOVA) and post-hoc Tukey tests (P<.05). Film and phosphor plate radiopacity values were compared using simple regression analysisResults: Excellent linear correlation was found between film and phosphor plates for both composite resins and bonding agents. The composite materials Spectrum Tph and Natural Look exhibited the highest radiopacity with film and with phosphor plates, respectively. All the dentin bonding agents tested exhibited lower radiopacity than dentin. Conclusion: Synergy, Ice, Filtek Silorane, Filtek Z250, Clearfil Majesty Posterior, Herculite Classic, Spectrum Tph, and Natural Look composite materials exhibited greater radiopacity than dentin, and all the dentin bonding agents tested exhibited lower radiopacity than either enamel or dentin. (Eur J Dent 2012;6:115-122)


Author(s):  
Abdülkadir Şengün ◽  
Muhammet Yalçın ◽  
Hayriye Esra Ülker ◽  
Bora Öztürk ◽  
Sema S. Hakkı

1988 ◽  
Vol 67 (2) ◽  
pp. 467-470 ◽  
Author(s):  
E.L. Pashley ◽  
L. Tao ◽  
J.R. Mackert ◽  
D.H. Pashlety

Dogs were utilized in a study to compare the bond strengths of dentin bonding agents made to dentin in vivo and then again in vitro in the same teeth 30 min, one day, one week, and one month post-extraction. No statistically significant differences were observed between bonds made in vivo and those made in vitro at any time period. Contamination of the dentin surfaces with blood or saliva lowered the bond strengths, but these could be restored to control values by re-surfacing of the dentin with a bur.


2010 ◽  
Vol 36 (11) ◽  
pp. 1844-1850 ◽  
Author(s):  
Na Ryoung Kim ◽  
Hee Chul Park ◽  
Insook Kim ◽  
Bum-Soon Lim ◽  
Hyeong-Cheol Yang

Sign in / Sign up

Export Citation Format

Share Document