Serological and virological detection of canine herpesvirus-1 in adult dogs with and without reproductive disorders

Abstract Background: Canine herpesvirus 1 (CHV-1) is known as a causal agent of death in newborn puppies and fertility problems in adults with a widespread distribution. There has been an increasing concern among dog breeders in Iran regarding CHV-1. This study is the first molecular detection of CHV-1 in breeding kennels and farm dogs in Iran using real-time PCR and investigation of various predisposing factors. Results: A total of 63 vaginal samples collected from 47 breeding kennels and 16 farm dogs were evaluated. In general, 21 out of 63 (33.3%) of vaginal samples were CHV-1-positive. The percentage of infection was higher in farm dogs, which was statistically significant. There was no significant association regarding other probable predisposing factors, including age, breed, pregnancy, and reproductive disorders. Conclusions: Considering the results of this study, CHV-1 is common in farm and breeding kennels and could pose a threat. Further studies are required to better understanding the distribution of the virus within Iran to advise dog breeders to more appropriate measures for management of CHV-1.

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The expression and serological reactivity of recombinant canine herpesvirus 1 glycoprotein D

Acta Veterinaria Brno ◽

10.2754/avb201685020113 ◽

2016 ◽

Vol 85 (2) ◽

pp. 113-119

Author(s):

MarkéŽta Vaňkov‡á ◽

Dobromila Molinková ◽

Vladimír Celer

Keyword(s):

Indirect Immunofluorescence ◽

Immunofluorescence Assay ◽

Indirect Immunofluorescence Assay ◽

Bacterial Cells ◽

Glycoprotein D ◽

Reproductive Disorders ◽

Recombinant Glycoprotein ◽

Canine Herpesvirus ◽

History Of ◽

Herpesvirus 1

The aim of this work was to express recombinant glycoprotein D ofcanine herpesvirus 1in bacterial cells and to evaluate its diagnostic sensitivity and specificity when compared to traditional serological methods. The gene fragment coding glycoprotein D ofcanine herpesvirus 1was amplified by polymerase chain reaction, cloned into plasmid vector and expressed inEscherichia colicells. Recombinant protein was then purified and used as an antigen in immunoblot for a detection ofcanine herpesvirus 1specific antibodies. Antibody testing was performed on the panel of 100 canine sera by immunoblot with recombinant glycoprotein D as antigen and compared with indirect immunofluorescence assay. Serum samples were collected from 83 dogs with no history ofcanine herpesvirus 1or reproductive disorders, and from 17 dogs from breeding kennels with a history ofcanine herpesvirus 1related reproductive disorders. Sensitivity of glycoprotein D based immunoblot was 89.2% and specificity was 93%. Kappa value was calculated to be 0.8 between immunoblot and indirect immunofluorescence assay. Antibodies againstcanine herpesvirus 1infection were detected in 33% of samples by immunoblot assay. Our study confirms that recombinant glycoprotein D expressed in bacterial cells could be used as a suitable and sensitive antigen for immunological tests and that herpesvirus infection seems to be common among the canine population in the Czech Republic.

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The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

10.21203/rs.2.16695/v1 ◽

2019 ◽

Author(s):

Mahdieh Rezaei ◽

Maziar Jajarmi ◽

Ramin Alizadeh ◽

Mohammad Khalili ◽

Homayoon Babaei

Keyword(s):

Neonatal Death ◽

Molecular Detection ◽

Definitive Diagnosis ◽

Molecular Assay ◽

Reproductive Disorders ◽

Viral Dna ◽

Canine Herpesvirus ◽

Management Measures ◽

Herpesvirus 1 ◽

Veterinary Hospital

Abstract Background Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran, not definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Samples were collected and evaluated using real-time PCR.Results Viral DNA was detected in 21 samples from a total of 140 (15%) collected samples which were related to 14 uterine samples (20%) and 7 (10%) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant.Conclusions This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

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