Detection of Canine herpesvirus 1 in the breeding kennel and farm dogs by Real-time PCR

Abstract Background: Canine herpesvirus 1 (CHV-1) is known as a causal agent of death in newborn puppies and fertility problems in adults with a widespread distribution. There has been an increasing concern among dog breeders in Iran regarding CHV-1. This study is the first molecular detection of CHV-1 in breeding kennels and farm dogs in Iran using real-time PCR and investigation of various predisposing factors. Results: A total of 63 vaginal samples collected from 47 breeding kennels and 16 farm dogs were evaluated. In general, 21 out of 63 (33.3%) of vaginal samples were CHV-1-positive. The percentage of infection was higher in farm dogs, which was statistically significant. There was no significant association regarding other probable predisposing factors, including age, breed, pregnancy, and reproductive disorders. Conclusions: Considering the results of this study, CHV-1 is common in farm and breeding kennels and could pose a threat. Further studies are required to better understanding the distribution of the virus within Iran to advise dog breeders to more appropriate measures for management of CHV-1.

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The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

10.21203/rs.2.16695/v1 ◽

2019 ◽

Author(s):

Mahdieh Rezaei ◽

Maziar Jajarmi ◽

Ramin Alizadeh ◽

Mohammad Khalili ◽

Homayoon Babaei

Keyword(s):

Neonatal Death ◽

Molecular Detection ◽

Definitive Diagnosis ◽

Molecular Assay ◽

Reproductive Disorders ◽

Viral Dna ◽

Canine Herpesvirus ◽

Management Measures ◽

Herpesvirus 1 ◽

Veterinary Hospital

Abstract Background Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran, not definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Samples were collected and evaluated using real-time PCR.Results Viral DNA was detected in 21 samples from a total of 140 (15%) collected samples which were related to 14 uterine samples (20%) and 7 (10%) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant.Conclusions This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

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Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0052 ◽

2014 ◽

Vol 17 (2) ◽

pp. 367-369 ◽

Cited By ~ 3

Author(s):

K. Rypula ◽

A. Kumala ◽

P. Lis ◽

K. Niemczuk ◽

K. Płoneczka-Janeczko ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

Complement Fixation Test ◽

Complement Fixation ◽

Fixation Test ◽

Reproductive Disorders ◽

Serum Samples ◽

Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

Animal Biotechnology ◽

10.1080/10495398.2016.1268620 ◽

2017 ◽

Vol 28 (4) ◽

pp. 248-252 ◽

Cited By ~ 1

Author(s):

Sachin S. Pawar ◽

Chetan D. Meshram ◽

Niraj K. Singh ◽

Mohini Saini ◽

B. P. Mishra ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Bovine Herpesvirus ◽

Wild Type ◽

Pcr Assay ◽

Bovine Herpesvirus 1 ◽

Glycoprotein E ◽

Herpesvirus 1

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Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae

Journal of Clinical Microbiology ◽

10.1128/jcm.01962-15 ◽

2015 ◽

Vol 53 (12) ◽

pp. 3935-3937 ◽

Cited By ~ 5

Author(s):

Daniel Golparian ◽

Stina Boräng ◽

Martin Sundqvist ◽

Magnus Unemo

Keyword(s):

Neisseria Gonorrhoeae ◽

Real Time ◽

Sensitivity And Specificity ◽

Real Time Pcr ◽

Molecular Detection ◽

Analytical Sensitivity ◽

Pcr Assay ◽

Content Type ◽

Dna Assay ◽

Supplementary Test

The new BD Max GC real-time PCR assay showed high clinical and analytical sensitivity and specificity. It can be an effective and accurate supplementary test for the BD ProbeTec GC Qx amplified DNA assay, which had suboptimal specificity, and might also be used for initial detection ofNeisseria gonorrhoeae.

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