The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

Abstract Background Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran, not definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Samples were collected and evaluated using real-time PCR.Results Viral DNA was detected in 21 samples from a total of 140 (15%) collected samples which were related to 14 uterine samples (20%) and 7 (10%) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant.Conclusions This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

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Detection of Canine herpesvirus 1 in the breeding kennel and farm dogs by Real-time PCR

10.21203/rs.3.rs-16008/v1 ◽

2020 ◽

Author(s):

Mahdieh Rezaei ◽

Maziar Jajarmi ◽

Saba Kamani ◽

Mohammad Khalili ◽

Homayoon Babaei

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Molecular Detection ◽

Predisposing Factors ◽

Reproductive Disorders ◽

Widespread Distribution ◽

Canine Herpesvirus ◽

Herpesvirus 1 ◽

Fertility Problems ◽

Breeding Kennel

Abstract Background: Canine herpesvirus 1 (CHV-1) is known as a causal agent of death in newborn puppies and fertility problems in adults with a widespread distribution. There has been an increasing concern among dog breeders in Iran regarding CHV-1. This study is the first molecular detection of CHV-1 in breeding kennels and farm dogs in Iran using real-time PCR and investigation of various predisposing factors. Results: A total of 63 vaginal samples collected from 47 breeding kennels and 16 farm dogs were evaluated. In general, 21 out of 63 (33.3%) of vaginal samples were CHV-1-positive. The percentage of infection was higher in farm dogs, which was statistically significant. There was no significant association regarding other probable predisposing factors, including age, breed, pregnancy, and reproductive disorders. Conclusions: Considering the results of this study, CHV-1 is common in farm and breeding kennels and could pose a threat. Further studies are required to better understanding the distribution of the virus within Iran to advise dog breeders to more appropriate measures for management of CHV-1.

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First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon

Journal of Veterinary Science ◽

10.4142/jvs.21202 ◽

2022 ◽

Vol 23 ◽

Author(s):

Marcela dos Santos Castro ◽

Maridelzira Betania Moraes David ◽

Evonnildo Costa Gonçalves ◽

Andrei Santos Siqueira ◽

Rodrigo Rodrigues Virgulino ◽

...

Keyword(s):

Molecular Detection ◽

Brazilian Amazon ◽

Canine Herpesvirus ◽

Herpesvirus 1

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Risk factors and reproductive disorders associated with canine herpesvirus-1 (CHV-1)

Theriogenology ◽

10.1016/s0093-691x(03)00249-8 ◽

2004 ◽

Vol 61 (4) ◽

pp. 619-636 ◽

Cited By ~ 27

Author(s):

Veerle Ronsse ◽

John Verstegen ◽

Karine Onclin ◽

Frédéric Farnir ◽

Hervé Poulet

Keyword(s):

Risk Factors ◽

Reproductive Disorders ◽

Canine Herpesvirus ◽

Herpesvirus 1

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Serological and virological detection of canine herpesvirus-1 in adult dogs with and without reproductive disorders

The Veterinary Journal ◽

10.1016/j.tvjl.2014.03.001 ◽

2014 ◽

Vol 200 (2) ◽

pp. 257-260 ◽

Cited By ~ 4

Author(s):

A. Pratelli ◽

V. Colao ◽

M. Losurdo

Keyword(s):

Reproductive Disorders ◽

Canine Herpesvirus ◽

Herpesvirus 1

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The expression and serological reactivity of recombinant canine herpesvirus 1 glycoprotein D

Acta Veterinaria Brno ◽

10.2754/avb201685020113 ◽

2016 ◽

Vol 85 (2) ◽

pp. 113-119

Author(s):

MarkéŽta Vaňkov‡á ◽

Dobromila Molinková ◽

Vladimír Celer

Keyword(s):

Indirect Immunofluorescence ◽

Immunofluorescence Assay ◽

Indirect Immunofluorescence Assay ◽

Bacterial Cells ◽

Glycoprotein D ◽

Reproductive Disorders ◽

Recombinant Glycoprotein ◽

Canine Herpesvirus ◽

History Of ◽

Herpesvirus 1

The aim of this work was to express recombinant glycoprotein D ofcanine herpesvirus 1in bacterial cells and to evaluate its diagnostic sensitivity and specificity when compared to traditional serological methods. The gene fragment coding glycoprotein D ofcanine herpesvirus 1was amplified by polymerase chain reaction, cloned into plasmid vector and expressed inEscherichia colicells. Recombinant protein was then purified and used as an antigen in immunoblot for a detection ofcanine herpesvirus 1specific antibodies. Antibody testing was performed on the panel of 100 canine sera by immunoblot with recombinant glycoprotein D as antigen and compared with indirect immunofluorescence assay. Serum samples were collected from 83 dogs with no history ofcanine herpesvirus 1or reproductive disorders, and from 17 dogs from breeding kennels with a history ofcanine herpesvirus 1related reproductive disorders. Sensitivity of glycoprotein D based immunoblot was 89.2% and specificity was 93%. Kappa value was calculated to be 0.8 between immunoblot and indirect immunofluorescence assay. Antibodies againstcanine herpesvirus 1infection were detected in 33% of samples by immunoblot assay. Our study confirms that recombinant glycoprotein D expressed in bacterial cells could be used as a suitable and sensitive antigen for immunological tests and that herpesvirus infection seems to be common among the canine population in the Czech Republic.

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Canine herpesvirus-1 (CHV-1): clinical, serological and virological patterns in breeding colonies

Theriogenology ◽

10.1016/j.theriogenology.2004.11.016 ◽

2005 ◽

Vol 64 (1) ◽

pp. 61-74 ◽

Cited By ~ 31

Author(s):

Veerle Ronsse ◽

John Verstegen ◽

Etienne Thiry ◽

Karine Onclin ◽

Christine Aeberlé ◽

...

Keyword(s):

Canine Herpesvirus ◽

Herpesvirus 1

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Experimental primary ocular canine herpesvirus-1 infection in adult dogs

American Journal of Veterinary Research ◽

10.2460/ajvr.70.6.740 ◽

2009 ◽

Vol 70 (6) ◽

pp. 740-740

Keyword(s):

Canine Herpesvirus ◽

Herpesvirus 1

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Attachment and Penetration of Canine Herpesvirus 1 in Non-Permissive Cells.

Journal of Veterinary Medical Science ◽

10.1292/jvms.62.965 ◽

2000 ◽

Vol 62 (9) ◽

pp. 965-970 ◽

Cited By ~ 7

Author(s):

Kazuo NAKAMICHI ◽

Kentaro OHARA ◽

Yasunobu MATSUMOTO ◽

Haruki OTSUKA

Keyword(s):

Canine Herpesvirus ◽

Herpesvirus 1

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Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000300011 ◽

2013 ◽

Vol 22 (3) ◽

pp. 385-390 ◽

Cited By ~ 25

Author(s):

Natasha Gandolfi Miceli ◽

Fernando Antonio Gavioli ◽

Luiz Ricardo Gonçalves ◽

Marcos Rogério André ◽

Valéria Régia Franco Sousa ◽

...

Keyword(s):

Molecular Detection ◽

Western Region ◽

Mato Grosso ◽

Animal Shelters ◽

Blood Samples ◽

Cytauxzoon Felis ◽

The City ◽

Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

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