Immunogenicity and protective efficacy in mice of a formaldehyde-inactivated Indian strain of Japanese encephalitis virus grown in Vero cells

Vaccine ◽  
2004 ◽  
Vol 22 (27-28) ◽  
pp. 3669-3675 ◽  
Author(s):  
Mohan Babu Appaiahgari ◽  
Sudhanshu Vrati
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Protective Efficacy ◽  
Vero Cells ◽  
Encephalitis Virus ◽  
Indian Strain

A purified inactivated Japanese encephalitis virus vaccine made in vero cells

Vaccine ◽  
2001 ◽  
Vol 19 (31) ◽  
pp. 4557-4565 ◽  
Author(s):  
Ashok K. Srivastava ◽  
J.Robert Putnak ◽  
Sung H. Lee ◽  
Sun P. Hong ◽  
Sang B. Moon ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Virus Vaccine ◽  
Vero Cells ◽  
Encephalitis Virus ◽  
Made In

scholarly journals Interference in Japanese encephalitis virus infection of Vero cells by a cationic amphiphilic drug, chlorpromazine

2003 ◽  
Vol 84 (7) ◽  
pp. 1737-1741 ◽  
Author(s):  
Masaru Nawa ◽  
Tomohiko Takasaki ◽  
Ken-Ichiro Yamada ◽  
Ichiro Kurane ◽  
Toshitaka Akatsuka
Keyword(s):  
Virus Infection ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Vero Cells ◽  
Encephalitis Virus ◽  
Japanese Encephalitis Virus Infection ◽  
Amphiphilic Drug

scholarly journals Complete protection for mice conferred by a DNA vaccine based on Japanese encephalitis virus P3 strain that is used to prepare the inactivated vaccine in China

2020 ◽  
Author(s):  
Ran Wang ◽  
Xiaozheng Yu ◽  
Yan Wang ◽  
Xiaoyan Zheng
Keyword(s):  
Japanese Encephalitis Virus ◽  
Dna Vaccine ◽  
Japanese Encephalitis ◽  
Protective Efficacy ◽  
Neutralizing Antibody ◽  
Encephalitis Virus ◽  
Inactivated Vaccine ◽  
Complete Protection ◽  
Igg Antibody ◽  
Humoral Immune

Abstract Background The incidence of Japanese encephalitis (JE) has been dramatically reduced in China after the coverage of the vaccine. It is believed that the live-attenuated Japanese encephalitis virus (JEV) vaccine SA14-14-2 has contributed a lot. Another vaccine that seems to have faded out of the public is an inactivated vaccine based on the JEV P3 strain, which is still considered to have certain modifiability, such as being transformed into a DNA vaccine to improve its immunogenicity. Methods In this study, the protective efficacy induced by a Japanese encephalitis DNA vaccine candidate pV-JP3ME encoding pre-membrane (prM) and envelope (E) proteins of P3 strain in BALB/c mice. The prM/E genes of the JEV P3 strain were subcloned into vector pVAX1 (pV) to construct pV-JP3ME. Results The plasmid DNA was immunized BALB/c mice, high titers of IgG antibody and neutralizing antibody (nAb) against JEV were detected. The key cytokines in splenocytes upon stimulation with JEV antigens were secreted. Finally, complete protective efficacy was generated after challenge with the JEV P3 strain in mice. Conclusions The DNA vaccine pV-JP3ME based on JEV P3 strain in this study can induce specific humoral immune and cytokine responses in mice, and provide complete protection for mice against JEV.

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