Tracking the spatiotemporal variability of the oxic–anoxic interface in the Baltic Sea with broadband acoustics

Anoxic zones, regions of the water column completely devoid of dissolved oxygen, occur in open oceans and coastal zones worldwide. The Baltic Sea is characterized by strong salinity-driven stratification, maintained by occasional water inflows from the Danish Straights and freshwater input from rivers. Between inflow events, the stratification interface between surface and deep waters hinders mixing and ventilation of deep water; consequently, the bottom waters of large regions of the Baltic are anoxic. The onset of the anoxic zone is closely coincident with the depth of the halocline and, as a result, the interface between oxic and anoxic waters corresponds to a strong impedance contrast. Here, we track acoustic scattering from the impedance contrast utilizing a broadband split-beam echosounder in the Western Gotland Basin and link it to a dissolved oxygen level of 2 ml/l using ground truth stations. The broadband acoustic dataset provides the means to remotely observe the spatiotemporal variations in the oxic–anoxic interface, map out the extent of the anoxic zone with high resolution, and identify several mechanisms influencing the vertical distribution of oxygen in the water column. The method described here can be used to study other systems with applications in ongoing oceanographic monitoring programs.

Impact of Cold Temperatures on Nitrogen Removal in Denitrifying Down-Flow Hanging Sponge (DDHS) Reactors

Innovative and low-energy solutions for the removal of nitrogen from domestic wastewater are needed to achieve regulatory ambitions. However, there is a lack of appropriate technologies for use in non-centralised applications, where receiving waterbodies also are potentially sensitive. Denitrifying down-flow hanging sponge (DDHS) reactors are a promising solution but their performance has not been assessed under colder operating conditions pertinent to northern climates. Two DDHS reactor configurations (short and tall anoxic zones) were tested under “typical” UK winter, summer, and spring/autumn temperatures. At 22 °C, both reactors achieved >58% total nitrogen (TN) removal from domestic wastewater with no significant differences in removal rates between configurations. However, denitrification was lost at 13 °C in the reactor with the short anoxic zone, and was lost totally in both systems at 6 °C. Efficient nitrification was retained at 6 °C in both reactors (>90% removal NH4–N), suggesting that while elevated TN removal was not retained under colder conditions, the DDHS systems still effectively removed ammonia under UK winter conditions. DDHS reactors show promise for use under colder temperature conditions, although optimisation is needed, including the derivation of temperature correction factors for nitrogen removal.

Impact of Bed Form Celerity on Oxygen Dynamics in the Hyporheic Zone

Oxygen distribution and uptake in the hyporheic zone regulate various redox-sensitive reactions and influence habitat conditions. Despite the fact that fine-grain sediments in streams and rivers are commonly in motion, most studies on biogeochemistry have focused on stagnant sediments. In order to evaluate the effect of bed form celerity on oxygen dynamics and uptake in sandy beds, we conducted experiments in a recirculating indoor flume. Oxygen distribution in the bed was measured under various celerities using 2D planar optodes. Bed morphodynamics were measured by a surface elevation sensor and time-lapse photography. Oxygenated zones in stationary beds had a conchoidal shape due to influx through the stoss side of the bed form, and upwelling anoxic water at the lee side. Increasing bed celerity resulted in the gradual disappearance of the upwelling anoxic zone and flattening of the interface between the oxic (moving fraction of the bed) and the anoxic zone (stationary fraction of the bed), as well as in a reduction of the volumetric oxygen uptake rates due shortened residence times in the hyporheic zone. These results suggest that including processes related to bed form migration are important for understanding the biogeochemistry of hyporheic zones.

Nitrous oxide emissions of a mesh separated single stage deammonification reactor

It is widely accepted that partial nitrification by ANAMMOX has the potential to become one of the key processes in wastewater treatment. However, large greenhouse gas emissions have been panobserved in many cases. A novel mesh separated reactor, developed to allow continuous operation of deammonification at smaller scale without external biomass selection, was compared to a conventional single-chamber deammonification sequencing batch reactor (SBR), where both were equally-sized pilot-scale reactors. The mesh reactor consisted of an aerated and an anoxic zone separated by a mesh. The resulting differences in the structure of the microbial community were detected by next-generation sequencing. When both systems were operated in a sequencing batch mode, both systems had comparable nitrous oxide emission factors in the range of 4% to 5% of the influent nitrogen load. A significant decrease was observed after switching from sequencing batch mode to continuous operation.

A research on the performance of down-flow hanging sponge (DHS) reactor treating domestic wastewater

The aim of this study was to evaluate the performance of a down-flow hanging sponge (DHS) system in treating domestic wastewater. A pilot-scale of DHS system with a capacity of 60 L was designed and fabricated from polyvinyl chloride (PVC). The dimensions of DHS system are 1.5 m in height and square surface with 0.2 m in width, consists of three identical segments connected vertically in series. Each segment was filled by polyurethane sphere containing sponge. The total area of sponge and polyurethane sphere was 3,300 m2 m-3, density at 150 kg m-3, void ratio at 90%. DHS system was operated at ambient temperature within 82 days and stepwise increased of organic f rate from 0.5 to 1 and 1.5 kg COD m-3 d-1. The results showed that, this system performed well throughout the operational period and achieve the maximum removal of COD, BOD5, NH4+-N, and TN as 80%, 83%, 65% and 60%. The effluent of wastewater from DHS system achieved the requirement for National technical regulation on domestic wastewater of Vietnam type B QCVN 14:2008/BTNMT. In conclusion, the performance of DHS system indicated a high potential for application in removing organic matter and converting nitrogen ammonia to nitrogen nitrate, however it did not perform well for the removal of total nitrogen, it is necessary to study further by providing an anoxic zone in the system to enhance the treatment of nutrient in wastewater.

The first tetrapod from the mid-Miocene Clarkia lagerstätte (Idaho, USA)

The Clarkia lagerstätte (Latah Formation) of Idaho is well known for its beautifully preserved plant fossils as well as a fauna of insects and fish. Here we present the first known tetrapod fossil from these deposits. This specimen, recovered from the lower anoxic zone of the beds, is preserved as a carbonaceous film of a partial skeleton associated with a partial lower incisor and some tooth fragments. The morphology of the teeth indicates that the first tetrapod reported from Clarkia is a rodent. Its skeletal morphology as well as its bunodont and brachydont dentition suggests that it is a member of the squirrel family (Sciuridae). It is a large specimen that cannot be assigned to a known genus. Instead, it appears to represent the first occurrence of a new taxon with particularly gracile postcranial morphology likely indicative of an arboreal ecology. This new specimen is a rare glimpse into the poorly known arboreal mammal fossil record of the Neogene. It supports a greater taxonomic and ecological diversity of Miocene Sciuridae than previously recognized and offers new lines of inquiry in the paleoecological research enabled by the unique preservation conditions of the Clarkia biota.

Isoprenoid Quinones Resolve the Stratification of Redox Processes in a Biogeochemical Continuum from the Photic Zone to Deep Anoxic Sediments of the Black Sea

ABSTRACTThe stratified water column of the Black Sea serves as a model ecosystem for studying the interactions of microorganisms with major biogeochemical cycles. Here, we provide detailed analysis of isoprenoid quinones to study microbial redox processes in the ocean. In a continuum from the photic zone through the chemocline into deep anoxic sediments of the southern Black Sea, diagnostic quinones and inorganic geochemical parameters indicate niche segregation between redox processes and corresponding shifts in microbial community composition. Quinones specific for oxygenic photosynthesis and aerobic respiration dominate oxic waters, while quinones associated with thaumarchaeal ammonia oxidation and bacterial methanotrophy, respectively, dominate a narrow interval in suboxic waters. Quinone distributions indicate highest metabolic diversity within the anoxic zone, with anoxygenic photosynthesis being a major process in its photic layer. In the dark anoxic layer, quinone profiles indicate the occurrence of bacterial sulfur and nitrogen cycling, archaeal methanogenesis, and archaeal methanotrophy. Multiple novel ubiquinone isomers, possibly originating from unidentified intra-aerobic anaerobes, occur in this zone. The respiration modes found in the anoxic zone continue into shallow subsurface sediments, but quinone abundances rapidly decrease within the upper 50 cm below the sea floor, reflecting the transition to lower energy availability. In the deep subseafloor sediments, quinone distributions and geochemical profiles indicate archaeal methanogenesis/methanotrophy and potentially bacterial fermentative metabolisms. We observed that sedimentary quinone distributions track lithology, which supports prior hypotheses that deep biosphere community composition and metabolisms are determined by environmental conditions during sediment deposition.IMPORTANCEMicroorganisms play crucial roles in global biogeochemical cycles, yet we have only a fragmentary understanding of the diversity of microorganisms and their metabolisms, as the majority remains uncultured. Thus, culture-independent approaches are critical for determining microbial diversity and active metabolic processes. In order to resolve the stratification of microbial communities in the Black Sea, we comprehensively analyzed redox process-specific isoprenoid quinone biomarkers in a unique continuous record from the photic zone through the chemocline into anoxic subsurface sediments. We describe an unprecedented quinone diversity that allowed us to detect distinct biogeochemical processes, including oxygenic photosynthesis, archaeal ammonia oxidation, aerobic methanotrophy, and anoxygenic photosynthesis in defined geochemical zones.