Comparative studies on the properties of the extrinsic manganese-stabilizing protein from higher plants and of a synthetic peptide of its C-terminus

Heterotrimeric G proteins are important molecules for regulating plant architecture and transmitting external signals to intracellular target proteins in higher plants and mammals. The rice genome contains one canonical α subunit gene (RGA1), four extra-large GTP-binding protein genes (XLGs), one canonical β subunit gene (RGB1), and five γ subunit genes (tentatively named RGG1, RGG2, RGG3/GS3/Mi/OsGGC1, RGG4/DEP1/DN1/OsGGC3, and RGG5/OsGGC2). RGG1 encodes the canonical γ subunit; RGG2 encodes the plant-specific type of γ subunit with additional amino acid residues at the N-terminus; and the remaining three γ subunit genes encode the atypical γ subunits with cysteine abundance at the C-terminus. We aimed to identify the RGG3/GS3/Mi/OsGGC1 gene product, Gγ3, in rice tissues using the anti-Gγ3 domain antibody. We also analyzed the truncated protein, Gγ3∆Cys, in the RGG3/GS3/Mi/OsGGC1 mutant, Mi, using the anti-Gγ3 domain antibody. Based on nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, the immunoprecipitated Gγ3 candidates were confirmed to be Gγ3. Similar to α (Gα) and β subunits (Gβ), Gγ3 was enriched in the plasma membrane fraction, and accumulated in the flower tissues. As RGG3/GS3/Mi/OsGGC1 mutants show the characteristic phenotype in flowers and consequently in seeds, the tissues that accumulated Gγ3 corresponded to the abnormal tissues observed in RGG3/GS3/Mi/OsGGC1 mutants.

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Comparative Studies of Herbicide Resistant Mutants of Synechocystis 6714 and of Higher Plants

Current Research in Photosynthesis ◽

10.1007/978-94-009-0511-5_124 ◽

1990 ◽

pp. 543-546 ◽

Cited By ~ 1

Author(s):

A.-L. Etienne ◽

C. Astier ◽

G. Ajlani ◽

D. Kirilovsky ◽

J.-M. Ducruet ◽

...

Keyword(s):

Comparative Studies ◽

Higher Plants ◽

Herbicide Resistant ◽

Resistant Mutants

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COMPARATIVE STUDIES ON PHOTOSYNTHESIS IN HIGHER PLANTS

Photophysiology ◽

10.1016/b978-0-12-282608-5.50007-2 ◽

1973 ◽

pp. 1-63 ◽

Cited By ~ 57

Author(s):

Olle Björkman

Keyword(s):

Comparative Studies ◽

Higher Plants

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THE INTERACTION OF HIGHER PLANTS AND SOIL MICRO-ORGANISMS: II. STUDY OF THE MICROBIAL POPULATION OF THE RHIZOSPHERE IN RELATION TO RESISTANCE OF PLANTS TO SOIL-BORNE DISEASES

Canadian Journal of Research ◽

10.1139/cjr40c-041 ◽

1940 ◽

Vol 18c (9) ◽

pp. 444-455 ◽

Cited By ~ 30

Author(s):

M. I. Timonin

Keyword(s):

Small Proportion ◽

Comparative Studies ◽

Root Rot ◽

Microbial Population ◽

Higher Plants ◽

General Trend ◽

Fertilizer Treatment ◽

Plating Method ◽

Rhizosphere Population ◽

Micro Organisms

Comparative studies of flax varieties resistant and susceptible respectively to wilt, and of tobacco varieties resistant and susceptible to black root rot, showed higher numbers of micro-organisms in the rhizosphere of the susceptible than of corresponding resistant plants. Though plants of the same variety showed considerable variation in rhizosphere population under field and greenhouse conditions, the general trend remained the same. The abundance of micro-organisms in the rhizosphere of plants of the same variety grown in plots receiving different fertilizer treatment showed relatively little difference, even though the soils varied greatly in productivity.Numbers of micro-organisms in the rhizosphere of flax were greater when the water content of the soil was maintained at 30%, than when held at 60%, of total moisture-holding capacity. However, the microbial population in the soil distant from the roots was lower in the drier soil.Differential counts of fungi and actinomycetes indicated that the number of colonies developing from spores or conidia comprised a small proportion of the total count. Sporulation of fungi was more profuse in soil distant from the plant than in the rhizosphere.The contact slide method indicated a greater number of micro-organisms in the rhizosphere than in soil distant from the roots and showed differences between the rhizosphere of resistant and susceptible varieties which agreed with results from the plating method.

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