Inhibition by guanosine cyclic monophosphate (cGMP) analogues of uptake of [3H]3′,5′-cGMP without stimulation of ATPase activity in human erythrocyte inside-out vesicles11Abbreviations: 3′,5′-cGMP, guanosine 3′,5′-cyclic monophosphate; 2′,3′-cGMP, guanosine 2′,3′-cyclic monophosphate; N-mb-cGMP, N2-monobutyryl guanosine 3′,5′-cyclic monophosphate; O-mb-cGMP, 2′-O-monobutyryl guanosine 3′,5′-cyclic monophosphate; Db-cGMP, N2,2′-O-dibutyryl guanosine 3′,5′-cyclic monophosphate; Br-cGMP, 8′-bromo guanosine 3′,5′-cyclic monophosphate; Rp-cGMPS, Rp-monophosphorothioate guanosine 3′,5′-cyclic monophosphate; Sp-cGMPS, Sp-monophosphorothioate guanosine 3′,5′-cyclic monophosphate; 3′,5′-cAMP, Adenosine 3′,5′-cyclic monophosphate; and MRP, multidrug resistance protein.

2001 ◽  
Vol 62 (4) ◽  
pp. 425-429 ◽  
Author(s):  
Emmanuel Boadu ◽  
Svanhild Vaskinn ◽  
Elisabeth Sundkvist ◽  
Ragnhild Jaeger ◽  
Georg Sager
Keyword(s):  
Multidrug Resistance ◽  
Atpase Activity ◽  
Human Erythrocyte ◽  
Multidrug Resistance Protein ◽  
Cyclic Monophosphate ◽  
Resistance Protein ◽  
Inside Out ◽  
Stimulation Of

scholarly journals Altered Drug-stimulated ATPase Activity in Mutants of the Human Multidrug Resistance Protein

1996 ◽  
Vol 271 (4) ◽  
pp. 1877-1883 ◽  
Author(s):  
Marianna Müller ◽  
Éva Bakos ◽  
Ervin Welker ◽  
András Váradi ◽  
Ursula A. Germann ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Atpase Activity ◽  
Multidrug Resistance Protein ◽  
Resistance Protein

scholarly journals Activation of Adenylyl Cyclase Causes Stimulation of Adenosine Receptors

2018 ◽  
Vol 45 (6) ◽  
pp. 2516-2528 ◽  
Author(s):  
Thomas Pleli ◽  
Antonia Mondorf ◽  
Nerea Ferreiros ◽  
Dominique Thomas ◽  
Karel Dvorak ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Adenylyl Cyclase ◽  
Cholera Toxin ◽  
Adenosine Receptors ◽  
Intracellular Camp ◽  
Multidrug Resistance Protein ◽  
Camp Concentration ◽  
Resistance Protein ◽  
Intracellular Camp Concentration ◽  
Stimulation Of

Background/Aims: Signaling of Gs protein-coupled receptors (GsPCRs) is accomplished by stimulation of adenylyl cyclase, causing an increase of the intracellular cAMP concentration, activation of the intracellular cAMP effectors protein kinase A (PKA) and Epac, and an efflux of cAMP, the function of which is still unclear. Methods: Activation of adenylyl cyclase by GsPCR agonists or cholera toxin was monitored by measurement of the intracellular cAMP concentration by ELISA, anti-phospho-PKA substrate motif phosphorylation by immunoblotting, and an Epac-FRET assay in the presence and absence of adenosine receptor antagonists or ecto-nucleotide phosphodiesterase/pyrophosphatase2 (eNPP2) inhibitors. The production of AMP from cAMP by recombinant eNPP2 was measured by HPLC. Extracellular adenosine was determined by LC-MS/MS, extracellular ATP by luciferase and LC-MS/MS. The expression of eNPP isoenzymes 1-3 was examined by RT-PCR. The expression of multidrug resistance protein 4 was suppressed by siRNA. Results: Here we show that the activation of GsPCRs and the GsPCRs-independent activation of Gs proteins and adenylyl cyclase by cholera toxin induce stimulation of cell surface adenosine receptors (A2A or A2B adenosine receptors). In PC12 cells stimulation of adenylyl cyclase by GsPCR or cholera toxin caused activation of A2A adenosine receptors by an autocrine signaling pathway involving cAMP efflux through multidrug resistance protein 4 and hydrolysis of released cAMP to AMP by eNPP2. In contrast, in PC3 cells cholera toxin- and GsPCR-induced stimulation of adenylyl cyclase resulted in the activation of A2B adenosine receptors. Conclusion: Our findings show that stimulation of adenylyl cyclase causes a remarkable activation of cell surface adenosine receptors.

scholarly journals Modulation by (iso)flavonoids of the ATPase activity of the multidrug resistance protein

FEBS Letters ◽  
1997 ◽  
Vol 413 (2) ◽  
pp. 344-348 ◽  
Author(s):  
J.H Hooijberg ◽  
H.J Broxterman ◽  
M Heijn ◽  
D.L.A Fles ◽  
J Lankelma ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Atpase Activity ◽  
Multidrug Resistance Protein ◽  
Resistance Protein
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