scholarly journals Interresidual Distance Determination by Four-Pulse Double Electron-Electron Resonance in an Integral Membrane Protein: The Na+/Proline Transporter PutP of Escherichia coli

2004 ◽  
Vol 86 (4) ◽  
pp. 2551-2557 ◽  
Author(s):  
Gunnar Jeschke ◽  
Christoph Wegener ◽  
Monika Nietschke ◽  
Heinrich Jung ◽  
Heinz-Jürgen Steinhoff
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Integral Membrane Protein ◽  
Distance Determination ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

Distance Determination in Proteins insideXenopus laevisOocytes by Double Electron−Electron Resonance Experiments

2010 ◽  
Vol 132 (24) ◽  
pp. 8228-8229 ◽  
Author(s):  
Ryuji Igarashi ◽  
Tomomi Sakai ◽  
Hideyuki Hara ◽  
Takeshi Tenno ◽  
Toshiaki Tanaka ◽  
...  
Keyword(s):  
Distance Determination ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals Determining Oligomeric Order of a Membrane Protein by Double Electron-Electron Resonance Spectroscopy

2015 ◽  
Vol 108 (2) ◽  
pp. 93a
Author(s):  
Sergey Milikisiyants ◽  
Shenlin Wang ◽  
Rachel Munro ◽  
Matthew Donohue ◽  
Leonid S. Brown ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Resonance Spectroscopy ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

Direct Observation of Chain Lengths and Conformations in Oligofluorene Distributions from Controlled Polymerization by Double Electron-Electron Resonance

2019 ◽  
Author(s):  
Dennis Bücker ◽  
Annika Sickinger ◽  
Julian D. Ruiz Perez ◽  
Manuel Oestringer ◽  
Stefan Mecking ◽  
...  
Keyword(s):  
Chain Length ◽  
Length Distribution ◽  
Catalyst System ◽  
Chain Conformation ◽  
Controlled Polymerization ◽  
Chain Length Distribution ◽  
Electron Resonance ◽  
Double Electron ◽  
The Individual ◽  
Double Electron Electron Resonance

Synthetic polymers are mixtures of different length chains, and their chain length and chain conformation is often experimentally characterized by ensemble averages. We demonstrate that Double-Electron-Electron-Resonance (DEER) spectroscopy can reveal the chain length distribution, and chain conformation and flexibility of the individual n-mers in oligo-(9,9-dioctylfluorene) from controlled Suzuki-Miyaura Coupling Polymerization (cSMCP). The required spin-labeled chain ends were introduced efficiently via a TEMPO-substituted initiator and chain terminating agent, respectively, with an in situ catalyst system. Individual precise chain length oligomers as reference materials were obtained by a stepwise approach. Chain length distribution, chain conformation and flexibility can also be accessed within poly(fluorene) nanoparticles.

In-Cell Double Electron–Electron Resonance at Nanomolar Protein Concentrations

2021 ◽  
pp. 3679-3684
Author(s):  
Svetlana Kucher ◽  
Christina Elsner ◽  
Mariya Safonova ◽  
Stefano Maffini ◽  
Enrica Bordignon
Keyword(s):  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance
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