A novel monoclonal stool antigen test for the detection of H. pylori before and after eradication therapy: A prospective study in outpatients

Background. Membranous nephropathy (MN) is a common cause of nephrotic syndrome. In most cases it is idiopathic, while it may also be secondary to many diseases. In this study, prevalence ofH. pyloriinfection and the effects ofH. pylorieradication on proteinuria levels were investigated.Methods. Thirty five patients with MN (19 male), 12 patients with IgA nephropathy (4 male) and 12 patients with focal segmental glomerulosclerosis (FSGS) (8 male) were studied. The presence ofH. pyloriantigen was investigated in renal tissues obtained by biopsy, and the effects ofH. pylorieradication on proteinuria levels were investigated.Results. Immunohistochemistry withH. pyloriantigen revealed no positive staining in the glomeruli of all patients. 19 patients (54%) with MN, 10 (83%) with IgA nephropathy and 4 (33%) with FSGS were positive forH. pyloristool antigen test(P=0.045). Patients withH. pyloriinfection were administered eradication therapy (lansoprazole, 30 mg twice daily, plus amoxicillin, 0.75 g twice daily, plus clarithromycin, 250 mg twice daily, for 14 days). Before the eradication therapy the mean proteinuria of patients with MN, IgA nephropathy and FSGS were 2.42 ± 3.24 g/day, 2.12 ± 1.63 g/day and 1.80 ± 1.32 g/day, respectively. Three months after eradication, baseline proteinuria levels of patients with MN significantly decreased to 1.26 ± 1.73 g/day(P=0.031). In all three groups there were no significant differences with regard to serum creatinine, albumin and C-reactive protein levels before and after eradication therapy.Conclusions. The eradication ofH. pyloriinfection may be effective to reduce proteinuria in patients with MN, while spontaneous remission of MN could not be excluded in this patient cohort. This trial is registered withNCT00983034.

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Sensitivity of a Novel Stool Antigen Test for Detection of Helicobacter pylori in Adult Outpatients before and after Eradication Therapy

Journal of Clinical Microbiology ◽

10.1128/jcm.42.3.1319-1321.2004 ◽

2004 ◽

Vol 42 (3) ◽

pp. 1319-1321 ◽

Cited By ~ 12

Author(s):

V. Weingart ◽

H. Russmann ◽

S. Koletzko ◽

J. Weingart ◽

W. Hochter ◽

...

Keyword(s):

Helicobacter Pylori ◽

Eradication Therapy ◽

Antigen Test ◽

Stool Antigen Test ◽

Before And After ◽

Stool Antigen

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Helicobacter pylori Stool Antigen Test: Results of a Prospective Study

Surgery Today ◽

10.1007/s00595-003-2718-9 ◽

2004 ◽

Vol 34 (4) ◽

pp. 318-322 ◽

Cited By ~ 5

Author(s):

Soykan Arikan ◽

Ahmet Kocakusak ◽

G�l Barut ◽

G�n�l Sengoz ◽

Ahmet F. Yucel ◽

...

Keyword(s):

Helicobacter Pylori ◽

Prospective Study ◽

Test Results ◽

Antigen Test ◽

Stool Antigen Test ◽

Stool Antigen ◽

A Prospective Study

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Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Children With Symptomatic Disease: A Prospective Study

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/00005176-200407000-00013 ◽

2004 ◽

Vol 39 (1) ◽

pp. 64-67 ◽

Cited By ~ 20

Author(s):

Yoram Elitsur ◽

Zandra Lawrence ◽

Ivor Hill

Keyword(s):

Helicobacter Pylori ◽

Prospective Study ◽

Helicobacter Pylori Infection ◽

Antigen Test ◽

Stool Antigen Test ◽

Symptomatic Disease ◽

Stool Antigen ◽

A Prospective Study

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The Use of 13C-Urea Breath Test for Non-Invasive Diagnosis of Helicobacter pylori Infection in Comparison to Endoscopy and Stool Antigen Test

Diagnostics ◽

10.3390/diagnostics10070448 ◽

2020 ◽

Vol 10 (7) ◽

pp. 448

Author(s):

Hamed Alzoubi ◽

Asma’a Al-Mnayyis ◽

Ibrahim Al rfoa ◽

Amin Aqel ◽

Mohammad Abu-Lubad ◽

...

Keyword(s):

Breath Test ◽

Eradication Therapy ◽

Urea Breath Test ◽

P Value ◽

Antigen Test ◽

Stool Antigen Test ◽

Non Invasive ◽

Stool Antigen ◽

13C Urea Breath Test ◽

H Pylori

Helicobacter pylori (H. pylori) can cause gastritis, peptic ulcer diseases and gastric carcinoma. Endoscopy as the gold standard method of diagnosis is an invasive procedure that might not be suitable in all scenarios. Therefore, this first study in Jordan aimed to assess the non-invasive 13C urea breath test (UBT) and stool antigen test for diagnosis of H. pylori infection and the successfulness of eradication therapy as alternatives for endoscopy. Hence, a total of 30 patients attending the endoscopy units at Alkarak teaching hospital were asked to complete a questionnaire with demographic and clinical data. They were then tested for H. pylori using 13C UBT and H. pylori stool antigen before having endoscopy. Another 30 patients who were positive for H. pylori by endoscopy were tested using both tests 6 weeks post eradication therapy. Results showed that the rate of H. pylori detection using endoscopy was 56.7% (17/30). Heartburns (82.3%, p value = 0.019), epigastric pain (88.2%, p value = 0.007) and vomiting (70.5%, p value = 0.02) were the most significant symptoms. Family history of peptic ulcer diseases was significantly associated with an increased risk for having a H. pylori positive result (p value = 0.02). Compared to endoscopy, the sensitivity of 13C UBT for the diagnosis of H. pylori was 94.1% (16/17), while it was 76.5% (13/17) for the stool antigen test. The specificity of both tests was equal (76.9%). However, the positive predictive and negative predictive values (84.2% and 90.9%) for 13C UBT were higher than those (81.3% and 71.4%) for the stool antigen test. The accuracy of 13C UBT was 86.7% compared to 76.7% for the stool antigen test. There was an 87% agreement (20 patients out of 23) between both tests when used to assess success of the eradication therapy. In conclusion, the 13C UBT was found to be more sensitive and accurate than the stool antigen test when used for diagnosis; furthermore, it has a comparable outcome to the stool antigen test in assessing the successfulness of the eradication treatment.

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