scholarly journals Application of multi-assay system in a real-time monitoring of secretory function in adrenal chromaffin cells with a cell bed perfusion technique

1990 ◽  
Vol 52 ◽  
pp. 63
Author(s):  
Konosuke Kumakura ◽  
Mica Ohara-Imaizumi ◽  
Hirohisa Satoh
Keyword(s):  
Real Time ◽  
Chromaffin Cells ◽  
Assay System ◽  
Secretory Function ◽  
Adrenal Chromaffin Cells ◽  
Real Time Monitoring ◽  
Perfusion Technique ◽  
A Cell ◽  
Adrenal Chromaffin

scholarly journals Effects of adenosine derivatives on the secretory function of adrenal chromaffin cells: A point of action for theophylline and caffeine

1982 ◽  
Vol 32 ◽  
pp. 74
Author(s):  
Konosuke Kumakura ◽  
Nobuyuki Sasakawa ◽  
Masataka Kikuno ◽  
Kazuo Kobayashi ◽  
Nobuyuki Oizumi ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Secretory Function ◽  
Adrenal Chromaffin Cells ◽  
Adenosine Derivatives ◽  
Adrenal Chromaffin

Secretory function of adrenal chromaffin cells cultured on polypyrrole films

Biomaterials ◽  
1996 ◽  
Vol 17 (20) ◽  
pp. 1971-1974 ◽  
Author(s):  
Takashi Aoki ◽  
Mikako Tanino ◽  
Kohei Sanui ◽  
Naoya Ogata ◽  
Konosuke Kumakura
Keyword(s):  
Chromaffin Cells ◽  
Secretory Function ◽  
Adrenal Chromaffin Cells ◽  
Polypyrrole Films ◽  
Adrenal Chromaffin ◽  
Cells Cultured

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

1975 ◽  
Vol 33 ◽  
pp. 318-319
Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates
Keyword(s):  
Chromaffin Cells ◽  
Sodium Phosphate ◽  
Ganglion Cells ◽  
Ultrastructural Level ◽  
Osmic Acid ◽  
Adrenal Chromaffin Cells ◽  
Potassium Iodate ◽  
Perfusion Fluid ◽  
New Zealand White Rabbits ◽  
Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

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