scholarly journals Concentration-dependent conformational transition of alpha-elastin in aqueous solution.

1978 ◽  
Vol 253 (9) ◽  
pp. 2893-2894
Author(s):  
A.M. Tamburro ◽  
V. Guantieri ◽  
D. Daga-Gordini ◽  
G. Abatangelo
Keyword(s):  
Aqueous Solution ◽  
Conformational Transition

scholarly journals Effect of pH on the Conformational Transition of Silk Fibroin in Aqueous Solution Monitored by Thioflavin-T Fluorescence

2021 ◽  
Author(s):  
Ben Jia ◽  
Lan Jia ◽  
Jingxin Zhu
Keyword(s):  
Aqueous Solution ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Conformational Transition ◽  
Thioflavin T ◽  
Random Coil ◽  
Fluorescence Dye ◽  
Assembly Behavior ◽  
Β Sheet

Abstract In this work, the potential application of the fluorescence dye Thioflavin-T (ThT), which can specifically bind to amyloid, as a powerful tool for monitoring secondary structure transitions of silk fibroin (SF) induced by pH was examined. Results showed that ThT emission intensities substantially increased when pH decreased from 6.8 to 4.8. This increase may be due to conformational transitions from random coil to β-sheet. The morphology and secondary structure of SF were also investigated via TEM, AFM and circular dichroism spectroscopy. The information obtained herein can be utilized not only for the development of convenient and efficient noninvasive method for monitoring the assembly behavior of SF in aqueous solution but also for in vitro fluorescence imaging.

EFFECT OF STORAGE TIME AND CONCENTRATION ON STRUCTURE OF REGENERATED SILK FIBROIN SOLUTION

2006 ◽  
Vol 20 (25n27) ◽  
pp. 3878-3883 ◽  
Author(s):  
FANG XIE ◽  
HUILI SHAO ◽  
XUECHAO HU
Keyword(s):  
Aqueous Solution ◽  
Silk Fibroin ◽  
Storage Time ◽  
Conformational Transition ◽  
Solution Concentration ◽  
Cd Spectroscopy ◽  
Random Coil ◽  
Conformational Transformation ◽  
Regenerated Silk Fibroin ◽  
Α Helix

Concentrated regenerated silk fibroin (RSF) aqueous solutions with concentration close to that of the native silk fibroin (15.5%, 25.5% and 31%) were prepared. The effect of storage time and concentration on the conformational transition of the concentrated RSF aqueous solution was studied by Raman spectroscopy and circular dichroism (CD) spectroscopy. At the same time, the conformational change of RSF aqueous solution in flowing state was also investigated. It was found that the conformation of silk fibroin was changed gradually from random coil/α-helix to β-sheet structure during the storage. And the conformational transformation was accelerated with the increasing of the RSF aqueous solution concentration. When the solution was in flowing state, the conformational transformation was also accelerated.

Aggregation and conformational transition in aqueous solution of a bombolitin III analogue containing a photoreactive side-chain group

Biopolymers ◽  
1997 ◽  
Vol 42 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Chiara Cabrele ◽  
Sabrina Furin ◽  
Marion Gurrath ◽  
Stefano Mammi ◽  
Elisabetta Schievano ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Conformational Transition ◽  
Side Chain ◽  
Chain Group
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