scholarly journals Purification and characterization of adenylate cyclase from Escherichia coli K12.

1983 ◽  
Vol 258 (6) ◽  
pp. 3750-3758
Author(s):  
J K Yang ◽  
W Epstein
Keyword(s):  
Escherichia Coli ◽  
Adenylate Cyclase ◽  
Purification And Characterization ◽  
Escherichia Coli K12

Purification and characterization of catalase HPII from Escherichia coli K12

1986 ◽  
Vol 64 (7) ◽  
pp. 638-646 ◽  
Author(s):  
Peter C. Loewen ◽  
Jacek Switala
Keyword(s):  
Escherichia Coli ◽  
Sodium Dodecyl ◽  
Purification And Characterization ◽  
Unusual Structure ◽  
Escherichia Coli K12 ◽  
Molecular Weights ◽  
Ph Range ◽  
Heme Cofactor ◽  
Native Molecular Weight

Catalase (hydroperoxidase II or HPII) of Escherichia coli K12 has been purified using a protocol that also allows the purification of the second catalase HPI in large amounts. The purified HPII was found to have equal amounts of two subunits with molecular weights of 90 000 and 92 000. Only a single 92 000 subunit was present in the immunoprecipitate created when HPII antiserum was added directly to a crude extract, suggesting that proteolysis was responsible for the smaller subunit. The apparent native molecular weight was determined to be 532 000, suggesting a hexamer structure for the enzyme, an unusual structure for a catalase. HPII was very stable, remaining maximally active over the pH range 4–11 and retaining activity even in a solution of 0.1% sodium dodecyl sulfate and 7 M urea. The heme cofactor associated with HPII was also unusual for a catalase, in resembling heme d (a2) both spectrally and in terms of solubility. On the basis of heme-associated iron, six heme groups were associated with each molecule of enzyme or one per subunit.

Purification and Characterization of the Periplasmic Nickel-Binding Protein NikA of Escherichia coli K12

1995 ◽  
Vol 227 (3) ◽  
pp. 857-865 ◽  
Author(s):  
Karinne Pina ◽  
Clarisse Navarro ◽  
Laura Mcwalter ◽  
David H. Boxer ◽  
Nicholas C. Price ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Purification And Characterization ◽  
Escherichia Coli K12 ◽  
Nickel Binding

scholarly journals Purification and Characterization of the Periplasmic Nickel-Binding Protein NikA of Escherichia coli K12

2008 ◽  
Vol 227 (3) ◽  
pp. 857-865 ◽  
Author(s):  
Karinne Pina ◽  
Clarisse Navarro ◽  
Laura Mcwalter ◽  
David H. Boxer ◽  
Nicholas C. Price ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Purification And Characterization ◽  
Escherichia Coli K12 ◽  
Nickel Binding
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