Characterization of lysyl residues of NADH-cytochrome b5 reductase implicated in charge-pairing with active-site carboxyl residues of cytochrome b5 by site-directed mutagenesis of an expression vector for the flavoprotein.

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

Characterization of the role of lysine 110 of NADH-cytochrome b5 reductase in the binding and oxidation of NADH by site-directed mutagenesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)88681-7 ◽

1992 ◽

Vol 267 (28) ◽

pp. 20164-20167

Author(s):

P Strittmatter ◽

J.M. Kittler ◽

J.E. Coghill

Keyword(s):

Cytochrome B5 ◽

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

Electrostatic interaction between NADH-cytochrome b5 reductase and cytochrome b5 studied by site-directed mutagenesis

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology ◽

10.1016/s0167-4838(97)00146-5 ◽

1998 ◽

Vol 1384 (1) ◽

pp. 16-22 ◽

Cited By ~ 16

Author(s):

Komei Shirabe ◽

Takushi Nagai ◽

Toshitsugu Yubisui ◽

Masazumi Takeshita

Keyword(s):

Electrostatic Interaction ◽

Cytochrome B5 ◽

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

STUDIES ON THE ROLE OF SERINE-127 IN NADH-CYTOCHROME b5 REDUCTASE BY SITE-DIRECTED MUTAGENESIS

Flavins and Flavoproteins 1990 ◽

10.1515/9783110855425-166 ◽

1991 ◽

pp. 855-858

Author(s):

T. Yubisui ◽

K. Shirabe ◽

M. Takeshita

Keyword(s):

Cytochrome B5 ◽

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

Role of cysteine residues in human NADH-cytochrome b5 reductase studied by site-directed mutagenesis. Cys-273 and Cys-283 are located close to the NADH-binding site but are not catalytically essential.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)89479-4 ◽

1991 ◽

Vol 266 (12) ◽

pp. 7531-7536

Author(s):

K Shirabe ◽

T Yubisui ◽

T Nishino ◽

M Takeshita

Keyword(s):

Binding Site ◽

Cytochrome B5 ◽

Cysteine Residues ◽

Directed Mutagenesis ◽

Cytochrome B5 Reductase ◽

Purification and Characterization of Microsomal Cytochrome b5 and NADH Cytochrome b5 Reductase from Pisum sativum

PLANT PHYSIOLOGY ◽

10.1104/pp.85.2.457 ◽

1987 ◽

Vol 85 (2) ◽

pp. 457-462 ◽

Cited By ~ 22

Author(s):

David R. Jollie ◽

Stephen G. Sligar ◽

Mary Schuler

Keyword(s):

Pisum Sativum ◽

Cytochrome B5 ◽

Purification And Characterization ◽

Microsomal Cytochrome ◽

Cytochrome B5 Reductase ◽

Microsomal Cytochrome B5 ◽

Characterization of the second form of NADH-Cytochrome b5 reductase gene from arachidonic acid-producing fungus Mortierella alpina 1S-4

Journal of Bioscience and Bioengineering ◽

10.1016/s1389-1723(00)87098-x ◽

1999 ◽

Vol 88 (6) ◽

pp. 667-671 ◽

Cited By ~ 16

Author(s):

Milan Certik ◽

Eiji Sakuradani ◽

Michihiko Kobayashi ◽

Sakayu Shimizu

Keyword(s):

Arachidonic Acid ◽

Cytochrome B5 ◽

Mortierella Alpina ◽

Cytochrome B5 Reductase ◽

Reductase Gene ◽

Purification and Partial Characterization of Microsomal NADH-Cytochrome b5 Reductase from Higher Plant Catharanthus roseus

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1993.2509 ◽

1993 ◽

Vol 197 (2) ◽

pp. 518-522 ◽

Cited By ~ 4

Author(s):

K.M. Madyastha ◽

N.K. Chary ◽

R. Holla ◽

T.B. Karegowdar

Keyword(s):

Catharanthus Roseus ◽

Cytochrome B5 ◽

Partial Characterization ◽

Higher Plant ◽

Cytochrome B5 Reductase ◽

Construction and Characterization of Mutant Water-Soluble PQQ Glucose Dehydrogenases with Altered Km Values—Site-Directed Mutagenesis Studies on the Putative Active Site

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1999.1157 ◽

1999 ◽

Vol 264 (3) ◽

pp. 820-824 ◽

Cited By ~ 37

Author(s):

Satoshi Igarashi ◽

Takafumi Ohtera ◽

Hiromi Yoshida ◽

Arief Budi Witarto ◽

Koji Sode

Keyword(s):

Active Site ◽

Water Soluble ◽

Directed Mutagenesis ◽

Putative Active Site

TOPOLOGICAL CHANGES IN THE CYP3A4 ACTIVE SITE PROBED WITH PHENYLDIAZENE: EFFECT OF INTERACTION WITH NADPH-CYTOCHROME P450 REDUCTASE AND CYTOCHROME B5 AND OF SITE-DIRECTED MUTAGENESIS

Drug Metabolism and Disposition ◽

10.1124/dmd.32.1.155 ◽

2004 ◽

Vol 32 (1) ◽

pp. 155-161 ◽

Cited By ~ 15

Author(s):

Yoshitaka Yamaguchi ◽

Kishore K. Khan ◽

You Ai He ◽

You Qun He ◽

James R. Halpert

Keyword(s):

Cytochrome P450 ◽

Active Site ◽

Cytochrome B5 ◽

Directed Mutagenesis ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Nadph Cytochrome P450 Reductase ◽

Topological Changes

Characterization of phosphate binding in the active site of barnase by site-directed mutagenesis and NMR

Biochemistry ◽

10.1021/bi00111a022 ◽

1991 ◽

Vol 30 (47) ◽

pp. 11348-11356 ◽

Cited By ~ 23

Author(s):

Elizabeth M. Meiering ◽

Mark Bycroft ◽

Alan R. Fersht

Keyword(s):

Active Site ◽

Directed Mutagenesis ◽

Phosphate Binding