scholarly journals Purification and Characterization of Microsomal Cytochrome b5 and NADH Cytochrome b5 Reductase from Pisum sativum

1987 ◽  
Vol 85 (2) ◽  
pp. 457-462 ◽  
Author(s):  
David R. Jollie ◽  
Stephen G. Sligar ◽  
Mary Schuler
Keyword(s):  
Pisum Sativum ◽  
Cytochrome B5 ◽  
Purification And Characterization ◽  
Microsomal Cytochrome ◽  
Cytochrome B5 Reductase ◽  
Microsomal Cytochrome B5 ◽  
Nadh Cytochrome

scholarly journals Isolation and characterization of the cytochrome domain of flavocytochrome b2 expressed independently in Escherichia coli

1992 ◽  
Vol 283 (1) ◽  
pp. 87-90 ◽  
Author(s):  
C E Brunt ◽  
M C Cox ◽  
A G P Thurgood ◽  
G R Moore ◽  
G A Reid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Field ◽  
Cytochrome B5 ◽  
Microsomal Cytochrome ◽  
E Coli ◽  
Flavocytochrome B2 ◽  
Isolation And Characterization ◽  
Exchange Rate Constant ◽  
Microsomal Cytochrome B5

The cytochrome domain of flavocytochrome b2 (L-lactate dehydrogenase) was expressed in the bacterium Escherichia coli and a purification procedure was developed. When expressed in E. coli, the b2-cytochrome domain contains protohaem IX and has an electronic absorption spectrum identical with that of the cytochrome b2 ‘core’ produced by proteolytic cleavage of the enzyme isolated from yeast. The b2-cytochrome domain isolated from E. coli has an Mr of 10,500 and a redox potential of -31 +/- 2 mV. High-field n.m.r. studies indicate pKa values for the haem propionate groups to be 4.8 and 4.6, consistent with these groups being exposed to solvent rather than buried inside the protein. Using n.m.r. spectroscopy, we have determined an electron self-exchange rate constant for the b2-cytochrome domain of 2.3 x 10(6) M-1.s-1, which is more than two orders of magnitude larger than the value obtained for microsomal cytochrome b5, a homologue of b2-cytochrome domain.

Characterization of a Novel Mutation in the NADH-Cytochrome b5 Reductase Gene Responsible for Rare Hereditary Methaemoglobinaemia Type I

2013 ◽  
Vol 130 (2) ◽  
pp. 122-125 ◽  
Author(s):  
Katarzyna Rawa ◽  
Liliana Chelmecka-Hanusiewicz ◽  
Danuta Plochocka ◽  
Katarzyna Pawinska-Wasikowska ◽  
Walentyna Balwierz ◽  
...  
Keyword(s):  
Novel Mutation ◽  
Cytochrome B5 ◽  
Type I ◽  
Cytochrome B5 Reductase ◽  
Reductase Gene ◽  
Nadh Cytochrome
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