scholarly journals Purification and Partial Characterization of Glucose 6-Phosphate Dehydrogenase from Neurospora crassa

1971 ◽  
Vol 246 (20) ◽  
pp. 6347-6352
Author(s):  
W.A. Scott ◽  
E.L. Tatum
Keyword(s):  
Neurospora Crassa ◽  
Partial Characterization ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals The Isolation and Partial Characterization of Two Novel Sphingolipids from Neurospora crassa: Di(Inositolphosphoryl)ceramide and [(gal)3glu]ceramide

1974 ◽  
Vol 249 (11) ◽  
pp. 3388-3394
Author(s):  
Robert L. Lester ◽  
Sharron W. Smith ◽  
Gerald B. Wells ◽  
Douglas C. Rees ◽  
Wallace W. Angus
Keyword(s):  
Neurospora Crassa ◽  
Partial Characterization

Genetic and biochemical studies of the hexose monophosphate shunt in Neurospora crassa. II. Characterization of biochemical defects of the morphological mutants colonial 2 and colonial 3

1971 ◽  
Vol 17 (6) ◽  
pp. 789-794 ◽  
Author(s):  
John F. Lechner ◽  
Kathyrn E. Fuscaldo ◽  
George Bazinet
Keyword(s):  
Neurospora Crassa ◽  
State Level ◽  
Hexose Monophosphate ◽  
Wild Type Enzyme ◽  
Hexose Monophosphate Shunt ◽  
Acid Dehydrogenase ◽  
Morphological Mutants ◽  
Altered Form ◽  
Glucose 6 Phosphate Dehydrogenase

The two dehydrogenases of the hexose monophosphate shunt, glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase, were examined in two morphology mutants of Neurospora crassa. Glucose-6-phosphate dehydrogenase extracted from the mutant colonial-2 was found in an altered form as compared to the wild-type enzyme. The second enzyme of the pathway was observed to be abnormal in the mutant colonial-3. In addition, 6-phosphogluconic acid dehydrogenase was found to exhibit non-classical kinetics. The data indicate that the altered morphology exhibited by both colonial-2 and colonial-3 is due to the suboptimal capacity of the hexose monophosphate shunt to function normally. This physiological impairment of the pathway is reflected in a lowered cellular steady-state level of NADP.

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