NUTRITIONAL STUDIES ON THE GROWTH OF FROG LARVÆ (RANA PIPIENS)

An electron microscope examination of fetal ovaries has revealed that developing germ cells are connected by intercellular bridges. In this investigation several species have been studied including human, mouse, chicken, and tadpole (Rana pipiens). These studies demonstrate that intercellular connections are similar in morphology regardless of the species.Basically, all bridges are characterized by a band of electron-dense material on the cytoplasmic side of the tri-laminar membrane surrounding the connection (Fig.l). This membrane is continuous with the plasma membrane of the conjoined cells. The dense material, however, never extends beyond the limits of the bridge. Variations in the configuration of intercellular connections were noted in all ovaries studied. However, the bridges in each individual species usually exhibits one structural characteristic seldom found in the others. For example, bridges in the human ovary very often have large blebs projecting from the lateral borders whereas the sides of the connections in the mouse gonad merely demonstrate a slight convexity.

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The application of video-enhanced constrast/differential interference constrast microscopy in conjunction with whole mount electron microscopy to the study of organelle transport

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102419 ◽

1988 ◽

Vol 46 ◽

pp. 66-67

Author(s):

J. H. Hayden

Keyword(s):

Electron Microscopy ◽

Rana Pipiens ◽

Silver Film ◽

Immunofluorescence Microscopy ◽

Organelle Transport ◽

Linear Element ◽

Whole Mount ◽

Carbon Coated ◽

Linear Elements ◽

Dic Microscopy

In a previous study, Allen video-enhanced constrast/differential interference constrast (AVEC-DIC) microscopy was used in conjunction with immunofluorescence microscopy to demonstrate that organelles and vesicle move in either direction along linear elements composed of microtubules. However, this study was limited in that the number of microtubules making up a linear element could not be determined. To overcome this limitation, we have used AVEC-DIC microscopy in conjunction with whole mount electron microscopy.Keratocytes from Rana pipiens were grown on glass coverslips as described elsewhere. Gold London Finder grids were Formvar- and carbon coated, and sterilized by exposure to ultraviolet light. It is important to select a Formvar film that gives a grey reflection when it is floated on water. A silver film is too thick and will detract from the image in the light microscope.

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A STUDY OF THE EFFECT OF SEVERAL EXPERIMENTAL CONDITIONS ON THE INTERMEDIN CONTENT AND CYTOCHEMICAL REACTIONS OF THE INTERMEDIATE LOBE OF THE FROG (RANA PIPIENS)

Acta Endocrinologica ◽

10.1530/acta.0.0230437 ◽

1956 ◽

Vol 23 (4) ◽

pp. 437-447 ◽

Cited By ~ 12

Author(s):

Robert Ortman

Keyword(s):

Rana Pipiens ◽

Intermediate Lobe ◽

Experimental Conditions

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Integrating nutritional studies into targeted floral provisioning forBombus impatiens

10.1603/ice.2016.113701 ◽

2016 ◽

Author(s):

Erin D Treanore

Keyword(s):

Nutritional Studies

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Nullisonmic‐Tetrasomic Wheat for Agronomic and Nutritional Studies 1

Crop Science ◽

10.2135/cropsci1978.0011183x001800020039x ◽

1978 ◽

Vol 18 (2) ◽

pp. 341-344 ◽

Cited By ~ 1

Author(s):

Branka Tucic ◽

P. E. McGuire ◽

C. O. Qualset ◽

D. D. Kasarda

Keyword(s):

Nutritional Studies

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NUTRITIONAL STUDIES ON SOME MARINE INTENSIVE FISH CULTURE TECHNIQUES

Menoufia Journal of Animal Poultry and Fish Production ◽

10.21608/mjapfp.2018.123929 ◽

2018 ◽

Vol 2 (3) ◽

pp. 59-60

Author(s):

D. M. S. EL- Saidy ◽

M. A. Essa ◽

A. M. A. Goda

Keyword(s):

Fish Culture ◽

Culture Techniques ◽

Intensive Fish Culture ◽

Nutritional Studies

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Long-Term Iron and Vitamin B12 Deficiency Are Present after Bariatric Surgery, Despite the Widespread Use of Supplements

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18094541 ◽

2021 ◽

Vol 18 (9) ◽

pp. 4541

Author(s):

Mauro Lombardo ◽

Arianna Franchi ◽

Roberto Biolcati Rinaldi ◽

Gianluca Rizzo ◽

Monica D’Adamo ◽

...

Keyword(s):

Bariatric Surgery ◽

Weight Loss ◽

Vitamin B12 ◽

Vitamin B12 Deficiency ◽

Ideal Body Weight ◽

Excess Weight ◽

Nutritional Deficiencies ◽

Average Percentage ◽

Nutritional Studies

There are few long-term nutritional studies in subjects undergoing bariatric surgery that have assessed weight regain and nutritional deficiencies. In this study, we report data 8 years after surgery on weight loss, use of dietary supplements and deficit of micronutrients in a cohort of patients from five centres in central and northern Italy. The study group consisted of 52 subjects (age: 38.1 ± 10.6 y, 42 females): 16 patients had Roux-en-Y gastric bypass (RYGB), 25 patients had sleeve gastrectomy (SG) and 11 subjects had adjustable gastric banding (AGB). All three bariatric procedures led to sustained weight loss: the average percentage excess weight loss, defined as weight loss divided by excess weight based on ideal body weight, was 60.6% ± 32.3. Despite good adherence to prescribed supplements, 80.7% of subjects (72.7%, AGB; 76.7%, SG; 93.8 %, RYGB) reported at least one nutritional deficiency: iron (F 64.3% vs. M 30%), vitamin B12 (F 16.6% vs. M 10%), calcium (F 33.3% vs. M 0%) and vitamin D (F 38.1% vs. M 60%). Long-term nutritional deficiencies were greater than the general population among men for iron and among women for vitamin B12.

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