scholarly journals Evidence for a calcium-sensitive factor which alters the alkaline pH sensitivity of sarcoplasmic reticulum calcium transport.

1981 ◽  
Vol 256 (6) ◽  
pp. 2934-2939
Author(s):  
C.A. Tate ◽  
A. Chu ◽  
J. McMillin-Wood ◽  
W.B. Van Winkle ◽  
M.L. Entman
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Transport ◽  
Ph Sensitivity ◽  
Alkaline Ph ◽  
Sensitive Factor ◽  
Sarcoplasmic Reticulum Calcium

Pressure Effects on the Interactions of the Sarcoplasmic Reticulum Calcium Transport Enzyme with Calcium and para-Nitrophenyl Phosphate

1987 ◽  
Vol 42 (5) ◽  
pp. 641-652 ◽  
Author(s):  
Wilhelm Hasselbach ◽  
Lore Stephan
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Transport ◽  
Activation Volume ◽  
Reaction Scheme ◽  
Pressure Effects ◽  
Calcium Dependent ◽  
Nitrophenyl Phosphate ◽  
Hydrolysis Of ◽  
Sarcoplasmic Reticulum Calcium ◽  
Effect Of Hydrostatic Pressure

The effect of hydrostatic pressure on calcium dependent p-nitrophenyl phosphate hydrolysis of the sarcoplasmic reticulum calcium transport enzyme has been investigated at different degree of enzyme saturation by calcium and Mg-p-nitrophenyl phosphate to distinguish between activation and binding volumes. The enzyme saturated by both ligands displays a significant dependence of the activation volume on pressure, rising from 20 ml/mol at atmospheric pressure (0.1 MPa) to 80 ml/mol at 100 MPa. At subsaturating concentration of Mg-p-nitrophenyl phosphate an activation volume of 35 ml/mol prevails between 0.1 and 40 MPa. At subsaturating concentration of calcium the activation volume approximates 80 ml/mol in the same pressure range. The binding volume for both substrates is likewise pressure dependent falling from 20 ml/mol to 0 ml/mol for Mg-p-nitrophenyl phosphate and rising from 67 ml/mol to 155 ml/mol for calcium. The pressure dependence of activation and binding volumes is analysed on account of a simplified reaction scheme yielding activation volumes and rate constants for individual reaction steps.

Altered sarcoplasmic reticulum calcium transport in the presence of the heavy metal chelator TPEN

Cell Calcium ◽  
2009 ◽  
Vol 46 (5-6) ◽  
pp. 347-355 ◽  
Author(s):  
Mónika Sztretye ◽  
János Almássy ◽  
Tamás Deli ◽  
Péter Szentesi ◽  
Carole Jung ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Sarcoplasmic Reticulum ◽  
Calcium Transport ◽  
Metal Chelator ◽  
Sarcoplasmic Reticulum Calcium
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