scholarly journals Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase.

1986 ◽  
Vol 261 (36) ◽  
pp. 17163-17169
Author(s):  
A Tzagoloff ◽  
M A Wu ◽  
M Crivellone
Keyword(s):  
Cytochrome C ◽  
Structural Gene ◽  
Mitochondrial Membrane ◽  
Core Protein ◽  
Membrane System ◽  
Cytochrome C Reductase ◽  
System Characterization

scholarly journals Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Tingting Yi ◽  
Xiaoxiao Wu ◽  
Zonghong Long ◽  
Guangyou Duan ◽  
Zhuoxi Wu ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Caspase 3 ◽  
Direct Evidence ◽  
Core Protein ◽  
Ischemia Reperfusion ◽  
Cardiac Cells ◽  
Protective Effects ◽  
Cytochrome C Reductase ◽  
Simulated Ischemia

In several recent studies, proteomics analyses suggest that increase of ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) is cardio-protective. However, direct evidence for this effect has not yet been obtained. Thus, the current study aimed to determine this effect and the mechanism underlying this effect. The results showed that overexpression of UQCRC1 protected H9c2 cardiac cells against in vitro simulated ischemia-reperfusion by maintaining mitochondrial membrane potential and suppressing the expression of caspase-3. These protective effects were significantly enhanced by exogenous Zn2+ but completely abolished by Zn2+-selective chelator TPEN. Furthermore, the upregulation of UQCRC1 reduced the concentration of free Zn2+ in mitochondria, whereas the downregulation of UQCRC1 increased the concentration of free Zn2+ in mitochondria. In conclusion, the overexpression of UQCRC1 can protect H9c2 cardiac cells against simulated ischemia/reperfusion, and this cardio-protective effect is likely mediated by zinc binding.

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