scholarly journals A high molecular weight DNA polymerase from Drosophila melanogaster embryos. Purification, structure, and partial characterization.

1979 ◽  
Vol 254 (19) ◽  
pp. 9886-9892
Author(s):  
G.R. Banks ◽  
J.A. Boezi ◽  
I.R. Lehman
Keyword(s):  
Molecular Weight ◽  
Drosophila Melanogaster ◽  
High Molecular Weight ◽  
Dna Polymerase ◽  
Partial Characterization ◽  
High Molecular Weight Dna

scholarly journals Cloning and characterization of the 5' part of the high molecular weight transcript of rat DNA polymerase beta.

1995 ◽  
Vol 42 (2) ◽  
pp. 253-258
Author(s):  
R Konopiński ◽  
R Nowak ◽  
J A Siedlecki
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Polymerase ◽  
Alternative Polyadenylation ◽  
Dna Polymerase Beta ◽  
High Molecular Weight Dna ◽  
Reading Frame ◽  
Polymerase Beta ◽  
Brain Cdna Library ◽  
Specific Regulation

A rat brain cDNA library has been constructed. Three identical clones of about 2.2 kb representing high molecular weight DNA polymerase beta transcript were found. Sequencing proved our earlier suggestion that both high and low molecular weight DNA polymerase beta transcripts have the same open reading frame and differ mainly at the 3' end. Because of that, alternative polyadenylation is discussed as a possible mechanism for tissue- and development-specific regulation of the DNA polymerase beta gene expression.

Isolation of high molecular weight DNA from wholeEuglena cells

PROTOPLASMA ◽  
1987 ◽  
Vol 141 (2-3) ◽  
pp. 139-148 ◽  
Author(s):  
Christine Mederic ◽  
Odile Bertaux ◽  
J. D. Rouzeau ◽  
R. Valencia
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Molecular Weight Dna

A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria)

1992 ◽  
Vol 38 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Ken F. Jarrell ◽  
David Faguy ◽  
Anne M. Hebert ◽  
Martin L. Kalmokoff
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Thermal Denaturation ◽  
Dna Isolation ◽  
Methanogenic Archaea ◽  
Restriction Enzymes ◽  
Mole Percent ◽  
High Molecular Weight Dna ◽  
Isolation Methods ◽  
General Method

High molecular weight DNA was readily isolated from all methanogens treated, as well as from thermophilic anaerobic eubacteria, by grinding cells frozen in liquid N2, prior to lysis with SDS. DNA can subsequently be purified by the usual phenol–chloroform extractions. The procedure yields DNA readily cut by restriction enzymes and suitable for oligonucleotide probing, as well as for mole percent G + C content determination by thermal denaturation. The method routinely yields DNA of high molecular weight and is an improvement over DNA isolation methods for many methanogens, which often involve an initial breakage of the cells in a French pressure cell. Key words: methanogens, archaebacteria, archaea, DNA isolation.

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