scholarly journals Purification and characterization of VDE, a site-specific endonuclease from the yeast Saccharomyces cerevisiae.

1993 ◽  
Vol 268 (29) ◽  
pp. 21844-21853
Author(s):  
F.S. Gimble ◽  
J Thorner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Purification And Characterization ◽  
Yeast Saccharomyces Cerevisiae ◽  
Site Specific ◽  
A Site

Purification and characterization of wheat α-gliadin synthesized in the yeast, Saccharomyces cerevisiae

Gene ◽  
1992 ◽  
Vol 116 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Ann E. Blechl ◽  
Kristin S. Thrasher ◽  
William H. Vensel ◽  
Frank C. Greene
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Purification And Characterization ◽  
Yeast Saccharomyces Cerevisiae

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

1987 ◽  
Vol 7 (6) ◽  
pp. 2087-2096
Author(s):  
B Sauer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Expression ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Leu2 Gene ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

scholarly journals Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (6) ◽  
pp. 2087-2096 ◽  
Author(s):  
B Sauer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Expression ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Leu2 Gene ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

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