Sensitive reversed-phase high-performance liquid chromatographic determination of aflatoxin M1 in dry milk

1986 ◽  
Vol 355 ◽  
pp. 340-344 ◽  
Author(s):  
Alessandro Carisano ◽  
Giancarlo della Torre
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Aflatoxin M1 ◽  
Dry Milk ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF CEFIXIME IN BULK DRUGS

2001 ◽  
Vol 24 (15) ◽  
pp. 2315-2324 ◽  
Author(s):  
Rolando González-Hernández ◽  
Lauro Nuevas-Paz ◽  
Laritza Soto-Mulet ◽  
Miguel López-López ◽  
Joseph Hoogmartens
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Liquid-chromatographic determination of 5-fluorocytosine.

1980 ◽  
Vol 26 (1) ◽  
pp. 117-119
Author(s):  
J O Miners ◽  
T Foenander ◽  
D J Birkett
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Therapeutic Monitoring ◽  
Pharmacokinetic Studies ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We report a "high-performance" liquid-chromatographic method for measuring 5-fluorocytosine in plasma and cerebrospinal fluid. After deproteinization with trichloroacetic acid, the supernates are chromatographed on a reversed-phase (C18) column. Response to concentration is linear in the range of 5 to 200 mg/L, with ultraviolet detection at 276 nm. The assay requires only 0.1 mL of plasma, is reproducible, and may be performed in less than 12 min. 5-Fluorocytosine concentrations determined by this procedure correlated well with those obtained by spectrofluorometry, although the present method is more specific with no observable interference from co-administered amphotericin B and most other commonly encountered drugs, including salicylat:. This method is applicable to the routine therapeutic monitoring of pediatric and adult patients as well as to pharmacokinetic studies.

Liquid-chromatographic determination of 4-aminopyridine in serum, saliva, and urine.

1981 ◽  
Vol 27 (3) ◽  
pp. 437-440 ◽  
Author(s):  
D R Uges ◽  
P Bouma
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Drug Concentrations ◽  
Liquid Chromatographic Determination ◽  
High Concentrations ◽  
Liquid Chromatographic

Abstract We have developed "high-performance" liquid-chromatographic methods for determining 4-aminopyridine, an acetylcholine-releasing drug, in serum, saliva, and urine. As little as 1 microgram/L can be detected by extracting the alkalinized sample plus the internal standard (3,4-diaminopyridine) into dichloromethane, mixing the organic phase with 1-pentanol, evaporating the dichloromethane, and injecting the residue onto a reversed-phase column, where it is eluted with acetonitrile/methanol/aqueous ammonium carbonate, with detection at 245 nm. Analytical recoveries from serum averaged 86.7%. The CV at 50 micrograms/L was 2.9% (n = 8). For urine samples containing very high concentrations of 4-aminopyridine, we mixed urine and potassium carbonate in an automatic injector vial, extracted the drug into dichloromethane, centrifuged, and injected an aliquot of the extract into the chromatograph. Analytical recoveries averaged 92%, and the CV was about 2% for drug concentrations of 0.1-8 mg/L of urine.

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