Analysis of fatty acids of human red cells without lipid extraction

Some of the properties of myelin forms prepared by freezing, thawing, and homogenization of ghosts of human red cells are described, among these being the "apparent molecular weight" as found by light scattering, the effect of repeated freezing and thawing on the "apparent molecular weight," the quantity of fatty acids split off at various temperatures of freezing, the effect of the anticoagulant used, and the remarkable constancy in the "apparent molecular weight" met with when myelin forms are prepared from the ghosts of the red cells of normal people. Taken together, these observations lead to the description of a "standard technique" for the preparations of myelin forms; this technique can later be extended to the myelin forms obtained from animals other than man and also to those obtained from abnormal red cells.

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Fatty acids in phospholipids isolated from human red cells

Lipids ◽

10.1007/bf02532542 ◽

1966 ◽

Vol 1 (6) ◽

pp. 391-398 ◽

Cited By ~ 32

Author(s):

J. H. Williams ◽

M. Kuchmak ◽

R. F. Witter

Keyword(s):

Fatty Acids ◽

Red Cells ◽

Human Red Cells

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The uptake of plasma fatty acids into human red cells and its relationship to cell age

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism ◽

10.1016/0005-2760(70)90213-4 ◽

1970 ◽

Vol 202 (1) ◽

pp. 9-20 ◽

Cited By ~ 12

Author(s):

Christine C. Winterbourn ◽

R.D. Batt

Keyword(s):

Fatty Acids ◽

Red Cells ◽

Plasma Fatty Acids ◽

Cell Age ◽

Human Red Cells

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In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651281 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 384-396 ◽

Cited By ~ 1

Author(s):

G Zbinden ◽

S Tomlin

Keyword(s):

Platelet Adhesion ◽

Sodium Citrate ◽

Blood Platelets ◽

In Vitro Assay ◽

Red Cells ◽

Platelet Adhesiveness ◽

Vitro Assay ◽

In Vitro System ◽

Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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