Observations on Inclusion Bodies in Pig Kidney Tissue Culture Cells Infected with Porcine Adenoviruses

1968 ◽  
Vol 9 (4) ◽  
pp. 300-305 ◽  
Author(s):  
J.B. Derbyshire ◽  
R.L. Chandler ◽  
K. Smith
Keyword(s):  
Tissue Culture ◽  
Inclusion Bodies ◽  
Kidney Tissue ◽  
Pig Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells

Formation of poliovirus in monkey kidney tissue culture cells

Virology ◽  
1962 ◽  
Vol 16 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Heather Donald Mayor ◽  
Liane E. Jordan
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells

Crystalline Arrays of Foot-and-Mouth Disease Virus in Tissue Culture Cells

1967 ◽  
Vol 25 ◽  
pp. 102-103
Author(s):  
S.S. Breese ◽  
J.H. Graves
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Disease Virus ◽  
Kidney Tissue ◽  
Foot And Mouth Disease ◽  
Bovine Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mouth Disease Virus ◽  
Swine Kidney

The formation of crystalline arrays of foot-and-mouth disease virus (FMDV) particles in swine kidney tissue culture cells has been examined in greater detail following the initial report of strain A119 in pig kidney cell line cultures, PK15. In the present experiments, FMDV strains recently isolated from field outbreaks in Argentina (A1, O2 and C3 CANEFA) were inoculated into primary swine kidney tissue cultures and samples at suitable intervals for embedding, sectioning and electron microscopy.The three strains of FMDV were stored at -20°C as the sixth bovine kidney tissue culture passage of original tongue tissue suspension. Immediately prior to use, they were inoculated into bovine kidney cultures and 1 ml of infected cell suspension was used in each swine kidney prescription bottle. After l/2 hr incubation at 37°C, 5 to 10 ml of maintenance medium was added and the bottles stored at 37°C. At intervals of 90 min, 3, 4, 5 and 6 hr after inoculation, bottles were processed for electron microscopy. Fluids were removed and the cells gently scraped from the glass into a few ml of Sorenson’s buffer at pH 7.2. Fixation was in 1% glutaraldehyde followed by 2% osmium tetroxide, dehydration, and embedment in epon. Sections were examined in an RCA-EMU-3-G microscope.

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

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