The Sexual Effect of Chicken Embryos on the Yolk Metabolites and Liver Lipid Metabolism

The metabolic processes of animals are usually affected by sex. Egg yolk is the major nutrient utilized for the growth and development of a chicken embryo. In this study, we explored the differences of yolk metabolites in male and female chicken embryos by LC–MS/MS. Furthermore, we investigated the mRNA expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS) in chicken embryo liver with different sexes in different embryonic stages. The results showed that the nutrient metabolites in the yolk of female chickens were mainly related to lipid metabolism and amino acid metabolism in the early embryonic stage, and vitamin metabolism in the late embryonic stage. The male yolk metabolites were mainly associated with lipid metabolism and nucleic acid metabolism in the early developmental stage, and amino acids metabolism in the late embryonic stage. There was no significant difference in the expression of LPL or FAS in livers of male and female chicken embryos at different embryonic stages. Our results may lead to a better understanding of the sexual effect on yolk nutrient metabolism during chicken embryonic development.

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

The effects of CEME and it complex with GO injected in ovo on the growth and development of chicken embryo hindlimb muscle were investigated. First, the preliminary in vitro study on primary muscle precursor cell culture obtained from a nine-day-old chicken embryo was performed to assess toxicity (MTT assay) of CEME, GO (100 ppm) and it complex with different concentrations (1, 2, 5, and 10 wt.%). The effect on cell proliferation was investigated by BrdU assay. CEME at concentrations 1–5% increased cell proliferation, but not the complex with GO. In vitro cytotoxicity was highest in 10% and GO groups. Next, the main experiment with chicken embryos was performed with CEME, GO and it complex injected in ovo on day one of embryogenesis. On day 20 of embryogenesis survival, morphological development, histological structure of the muscle, and biochemical parameters of blood serum of the embryos were measured. No negative effect on mortality, body weight, or biochemistry of blood after use of CEME or GO-CEME complexes was observed. Interestingly, the slight toxicity of GO, observed in in vitro studies, was not observed in vivo. The use of CEME at the levels of 2% and 5% improved the structure of the lower limb muscle by increasing the number of cells, and the administration of 2% CEME increased the number of nuclei visible in the stained cross-section of the muscle. The complex GO-CEME did not further improve the muscle structure. The results indicate that CEME can be applied as an in ovo enhancer of muscle development in broilers.

In Vitro Antiviral Activity of Green Tea Polyphenon-60 against Avian Paramyxoviruses

Avian paramyxoviruses (APMVs) have caused an economically significant drop in global domestic poultry production because of their high morbidity and mortality rates. Polyphenols are the major components of green tea that have great antiviral effects. This study aimed to evaluate the anti-APMV activities of polyphenon-60. Twelve APMV-1 strains representing three different pathotypes, two strains of APMV-2, one strain of APMV-3, and one strain of APMV-7 were propagated in chicken embryos. To determine the cytotoxic effect, chicken embryo fibroblasts were treated with the test compound in various concentrations. To assess the antiviral properties, time-dependent, dose-dependent, and virulence-dependent experiments were conducted in both cell and chicken embryo models. A reduction in virus titers was measured by the hemagglutination test. The inhibitory effect on virus adsorption to the chicken red blood cell (RBC) surface was examined by the hemagglutination inhibition test. The results showed that lentogenic and mesogenic APMV-1 strains, APMV-3 strain, and APMV-7 strain were significantly inhibited ( p < 0.05 ) by polyphenon-60 at 50 μg/ml, while the 50% cytotoxic concentration of the compound was 345 μg/ml. Polyphenon-60 also exhibited the inhibitory activity against hemagglutination by NDV. Taken together, the results suggest that polyphenon-60 has shown promise as an antiviral agent that has wide safety margins against APMVs, and challenge studies to evaluate its efficacy in chickens are necessary.

The Chicken Embryo Model: A Novel and Relevant Model for Immune-Based Studies

Dysregulation of the immune system is associated with many pathologies, including cardiovascular diseases, diabetes, and cancer. To date, the most commonly used models in biomedical research are rodents, and despite the various advantages they offer, their use also raises numerous drawbacks. Recently, another in vivo model, the chicken embryo and its chorioallantoic membrane, has re-emerged for various applications. This model has many benefits compared to other classical models, as it is cost-effective, time-efficient, and easier to use. In this review, we explain how the chicken embryo can be used as a model for immune-based studies, as it gradually develops an embryonic immune system, yet which is functionally similar to humans’. We mainly aim to describe the avian immune system, highlighting the differences and similarities with the human immune system, including the repertoire of lymphoid tissues, immune cells, and other key features. We also describe the general in ovo immune ontogeny. In conclusion, we expect that this review will help future studies better tailor their use of the chicken embryo model for testing specific experimental hypotheses or performing preclinical testing.

Teratogenic of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (TCDD) on Eye Diameters and Body Weight in Chicken Embryo

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a chemical compound resulting from the burning effect that is very dangerous for humans, plants, or animals which can cause skin damage, immunotoxic, hepatotoxic, carcinogenic, teratogenic, effects on reproduction, growth processes, neurobehavioral, and endocrine effects that are specific In addition, this compound can also reduce embryo weight and increase embryo mortality. This study aimed to determine the teratogenic of TCDD administration on eye diameters and body weight of chicken embryo. In this study there were 2 treatment groups, i.e: 1) P1 as a normal group without the addition of TCDD, and 2) P2 as a group with the addition of TCDD of 5 ng / egg with dissolved in 5 µL corn oil. The treatment was carried out for 7 days. The measurement data of eye diameters and body weight of chicken embryo were analyzed by the unpaired sample T test. The analysis showed that the teratogenic of TCDD had a significant effect on eye diameters and body weight of chicken embryos, where the teratogenic of TCDD caused a decrease in eye diameters and body weight loss in chicken embryos.

Thiacloprid Induced Developmental Neurotoxicity via ROS-Oxidative Injury and Inflammation in Chicken Embryo: The Possible Attenuating Role of Chicoric and Rosmarinic Acids

Insecticides are widely employed in agriculture to control pests and as major factors for enhancing crop productivity. Thiacloprid (TH) is one of the most-used insecticides worldwide. In this study, the negative impact of TH on the brain tissue of developing chicken embryo models and the modulatory effect of chicoric (CA) and rosmarinic (RA) acids were investigated. The eggs were injected in ovo with different doses of TH (0.1, 1, 10, and 100 μg/egg). TH significantly increased the oxidative damage in the brain of exposed embryos in a dose-dependent manner (p < 0.05). TH significantly elevated the oxidative stress markers; protein carbonyl, malondialdehyde content, and DNA damage (p < 0.05). Myeloperoxidase activity and nitric oxide significantly increased with overexpression of the pro-inflammatory cytokines (interferon gamma, tumor necrosis factor alpha, and interleukin-1 beta) and stress-related and apoptotic genes (NF-KB, Caspase-3) in the brain tissue on both biochemical and molecular levels (p < 0.05), while downregulating the expression of antiapoptotic Bcl-2. Co-treatment of CA and RA with TH markedly decreased the insecticide-induced toxicity with a prominent synergistic effect (p < 0.05). In conclusion, TH is suggested to be a possible neurotoxic to embryos of vertebrates including human. The study also revealed the antioxidant, anti-inflammatory, genoprotective, and antiapoptotic property of CA and RA against TH toxicity.