Microsomal monooxygenase activity in Tilapia (Oreochromis mossambicus) exposed to a bleached kraft mill effluent using different exposure systems

Chemosphere ◽  
2001 ◽  
Vol 45 (4-5) ◽  
pp. 581-588 ◽  
Author(s):  
Chien-Min Chen ◽  
Ming Chou Liu ◽  
Min Liang Shih ◽  
Shih-Ching Yu ◽  
Chia-Chien Yeh ◽  
...  
2010 ◽  
Vol 45 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Joanne L. Parrott ◽  
L. Mark Hewitt ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Pierre H. Martel ◽  
...  

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.


1986 ◽  
Vol 58 ◽  
pp. 79-94 ◽  
Author(s):  
Phillip A. Dean ◽  
Allan E. Rettie ◽  
Susan M. Turnblom ◽  
Moses J. Namkung ◽  
Mont R. Juchau

1977 ◽  
Vol 34 (6) ◽  
pp. 869-878 ◽  
Author(s):  
D. M. Whittle ◽  
K. W. Flood

Static and continuous-flow bioassays utilizing juvenile rainbow trout (Salmo gairdneri) were conducted on a northern Ontario bleached kraft mill effluent to assess the range of acute toxicity and related sublethal effects. The acute toxicity of the total mill effluent, as determined by static bioassay procedures, ranged from a 96-h LC50 value of 14–49.0% vol/vol. The results of these bioassays depended on testing methodology and effluent treatment systems employed by the mill when samples were collected. Continuous-flow bioassays conducted on the effluent Produced 96-h LC50 values of 21.8 and 24.8% vol/vol. Growth rate of juvenile rainbow trout (1–2 g) held under continuous-flow conditions for 18 days was significantly reduced at 6% vol/vol effluent concentration (P < 0.05). Flavor of yearling rainbow trout [Formula: see text] exposed to 3% effluent for 48 h was significantly impaired (P < 0.05). This effluent concentration, the lowest level impairing flavor represents approximately 0.12 of the 96-h LC50 derived from continuous-flow bioassays. No significant flavor impairment was detected in fish exposed for 144 h to a 2% effluent concentration Flavor impairment at a 3% vol/vol effluent concentration, the most sensitive sublethal response measured in this study, may also be the most critical response when assessing the economic impact of a kraft mill discharge to a freshwater ecosystem.


1981 ◽  
Vol 3 (12) ◽  
pp. 701-706 ◽  
Author(s):  
D. Livernoche ◽  
L. Jurasek ◽  
M. Desrochers ◽  
I. A. Veliky

1992 ◽  
Vol 11 (11) ◽  
pp. 1635-1651 ◽  
Author(s):  
Peter V. Hodson ◽  
Denis Thivierge ◽  
Marie-Claude Levesque ◽  
Michelle McWhirter ◽  
Karen Ralph ◽  
...  

1982 ◽  
Vol 242 (2) ◽  
pp. G147-G155 ◽  
Author(s):  
F. Hartmann ◽  
R. Owen ◽  
D. M. Bissell

Intestinal mucosal cells from the rat have been isolated by a new technique involving intravascular perfusion of an intestinal segment with collagenase. Detached cells were flushed from the intestinal lumen with a second perfusion circuit containing an oxygenated buffered solution with 1% bovine serum. Sequential collection of cells at intervals during the period of perfusion revealed that villus-tip cells are recovered first (after 15 min of collagenase perfusion), followed by midvillus (after 25 min) and lower villus cells (after 35 min). The isolated cells were judged intact and viable by the criteria of trypan blue dye exclusion, ultrastructural appearance, and metabolic activity. They were characterized as villus-tip, midvillus, and lower villus-crypt cells by their alkaline phosphatase and sucrase activity, glycoprotein formation, and [3H]thymidine incorporation. Microsomal monooxygenase activity was four to five times greater in villus-tip than in lower villus cells, whereas heme oxygenase exhibited a reverse gradient. The isolated cells synthesized heme and bilirubin under cell culture conditions.


2004 ◽  
Vol 50 (3) ◽  
pp. 11-20
Author(s):  
S.S. Helle ◽  
S.J.B. Duff

This study investigated the discrepancies between the BOD removal rates measured during short term assays and those measured during continuous activated sludge treatment of bleached kraft mill effluent (BKME). A combination of batch tests and fed batch tests with oxygen uptake rate (OUR), chemical oxygen demand (COD), biochemical oxygen demand (BOD), and mixed liquor volatile suspended solids (MLVSS) measurements were used to characterize the degradation rates for the activated sludge treatment of BKME and to divide the soluble readily biodegradable substrate into two to five separate fractions based on biodegradation rates. The removal rates varied by over an order of magnitude between the most readily degradable substrates (1 × 10-3 mg COD/mg MLVSS minute), and the more slowly degradable substrates (2 × 10-5 mg COD/mg MLVSS minute). If the readily biodegradable fraction of BKME was modeled as one substrate, initial rate kinetic measurements from batch tests were heavily influenced by the fractions with the greatest degradation rates, while any remaining BOD in the treated effluent was predominantly from the slowly degradable fraction, giving inconsistent results. Taking the multi-component nature of the wastewater into account, batch test results can be used to predict fed-batch and continuous activated sludge reactor performance.


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