Purification of Pseudomonas putida Branched-Chain Keto Acid Dehydrogenase E1 Component

2000 ◽  
pp. 129-138 ◽  
Author(s):  
Kathryn L. Hester ◽  
Jinhe Luo ◽  
John R. Sokatch
Keyword(s):  
Pseudomonas Putida ◽  
Keto Acid ◽  
Acid Dehydrogenase ◽  
Branched Chain

scholarly journals Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.

1987 ◽  
Vol 169 (4) ◽  
pp. 1619-1625 ◽  
Author(s):  
P J Sykes ◽  
G Burns ◽  
J Menard ◽  
K Hatter ◽  
J R Sokatch
Keyword(s):  
Pseudomonas Putida ◽  
Molecular Cloning ◽  
Keto Acid ◽  
Acid Dehydrogenase ◽  
Genes Encoding ◽  
Branched Chain

scholarly journals Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida andPseudomonas aeruginosa

2000 ◽  
Vol 182 (4) ◽  
pp. 1144-1149 ◽  
Author(s):  
Kathryn L. Hester ◽  
Jodi Lehman ◽  
Fares Najar ◽  
Lin Song ◽  
Bruce A. Roe ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Catabolite Repression ◽  
Carbon Sources ◽  
Keto Acid ◽  
Carbon Regulation ◽  
Acid Dehydrogenase ◽  
Transposon Mutants ◽  
Branched Chain ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of thebkd operon, were isolated and identified as crcand vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.

scholarly journals Purification of a branched-chain keto acid dehydrogenase from Pseudomonas putida.

1981 ◽  
Vol 148 (2) ◽  
pp. 647-652 ◽  
Author(s):  
J R Sokatch ◽  
V McCully ◽  
C M Roberts
Keyword(s):  
Pseudomonas Putida ◽  
Keto Acid ◽  
Acid Dehydrogenase ◽  
Branched Chain

scholarly journals Catabolite Repression Control by Crc in 2xYT Medium Is Mediated by Posttranscriptional Regulation of bkdR Expression in Pseudomonas putida

2000 ◽  
Vol 182 (4) ◽  
pp. 1150-1153 ◽  
Author(s):  
Kathryn L. Hester ◽  
K. T. Madhusudhan ◽  
John R. Sokatch
Keyword(s):  
Pseudomonas Putida ◽  
Catabolite Repression ◽  
Posttranscriptional Regulation ◽  
Keto Acid ◽  
Wild Type ◽  
Transcript Levels ◽  
Acid Dehydrogenase ◽  
Rich Media ◽  
Branched Chain ◽  
Control Protein

ABSTRACT The effect of growth in 2xYT medium on catabolite repression control in Pseudomonas putida has been investigated using the bkd operon, encoding branched-chain keto acid dehydrogenase. Crc (catabolite repression control protein) was shown to be responsible for repression of bkd operon transcription in 2xYT. BkdR levels were elevated in a P. putida crcmutant, but bkdR transcript levels were the same in both wild type and crc mutant. This suggests that the mechanism of catabolite repression control in rich media by Crc involves posttranscriptional regulation of the bkdR message.

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