Optimizing Luciferase Protein Fragment Complementation for Bioluminescent Imaging of Protein–Protein Interactions in Live Cells and Animals

Protein Fragment ◽

Luciferase Protein ◽

Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation

Methods in Molecular Biology - Genomics Protocols ◽

10.1007/978-1-59745-188-8_23 ◽

2008 ◽

pp. 339-352 ◽

Cited By ~ 15

Author(s):

Victor Villalobos ◽

Snehal Naik ◽

David Piwnica-Worms

Keyword(s):

Protein Interactions ◽

Firefly Luciferase ◽

Live Cells ◽

Protein Fragment ◽

Luciferase Protein ◽

Discovering Protein-Protein Interactions within the Programmed Cell Death Network Using a Protein-Fragment Complementation Screen

Cell Reports ◽

10.1016/j.celrep.2014.06.049 ◽

2014 ◽

Vol 8 (3) ◽

pp. 909-921 ◽

Cited By ~ 23

Author(s):

Yuval Gilad ◽

Ruth Shiloh ◽

Yaara Ber ◽

Shani Bialik ◽

Adi Kimchi

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Protein Interactions ◽

Protein Fragment ◽

Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot090068 ◽

2016 ◽

Vol 2016 (11) ◽

pp. pdb.prot090068 ◽

Cited By ~ 2

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Fragment ◽

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.tir117.000385 ◽

2017 ◽

Vol 17 (2) ◽

pp. 373-383 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Detection of Protein-Protein Interactions Using Protein-Fragment Complementation Assays (PCA)

Current Proteomics ◽

10.2174/157016407781387348 ◽

2007 ◽

Vol 4 (1) ◽

pp. 17-27 ◽

Cited By ~ 6

Author(s):

Emma Barnard ◽

Neil V. McFerran ◽

John Nelson ◽

David J. Timson

Keyword(s):

Protein Interactions ◽

Protein Fragment ◽

Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

Nature Protocols ◽

10.1038/nprot.2007.356 ◽

2007 ◽

Vol 2 (9) ◽

pp. 2302-2306 ◽

Cited By ~ 21

Author(s):

Ingrid Remy ◽

Galia Ghaddar ◽

Stephen W Michnick

Keyword(s):

Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

Nature Protocols ◽

10.1038/nprot.2007.266 ◽

2007 ◽

Vol 2 (9) ◽

pp. 2120-2125 ◽

Cited By ~ 40

Author(s):

Ingrid Remy ◽

F X Campbell-Valois ◽

Stephen W Michnick

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Corrigendum: Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.corr107815 ◽

2022 ◽

Vol 2022 (1) ◽

pp. pdb.corr107815

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Fragment ◽

Methods in Enzymology - Applications of Chimeric Genes and Hybrid Proteins - Part C: Protein-Protein Interactions and Genomics ◽

[14] Detection of protein-protein interactions by protein fragment complementation strategies

10.1016/s0076-6879(00)28399-7 ◽

2000 ◽

pp. 208-230 ◽

Cited By ~ 90

Author(s):

Stephen W. Michnick ◽

Ingrid Remy ◽

François-X. Campbell-Valois ◽

Alexis Vallée-Bélisle ◽

Joelle N. Pelletier

Keyword(s):

Protein Interactions ◽

Protein Fragment ◽

Extended linkers improve the detection of protein-protein interactions (PPIs) by dihydrofolate reductase protein-fragment complementation assay (DHFR PCA) in living cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.a117.000385 ◽

2018 ◽

Vol 17 (3) ◽

pp. 549-549 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽