212 Serological diagnosis of chagas disease: A confirmatory assay by western blot using preserved protein antigens of Trypanosoma cruzi

1996 ◽  
Vol 97 (1) ◽  
pp. 235-235
Author(s):  
R MENDES ◽  
S HOSHINOSHIMIZU ◽  
A MOURADASILVA ◽  
I MOTA ◽  
A OLUQUETTI ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Chagas Disease ◽  
Serological Diagnosis ◽  
Protein Antigens ◽  
Confirmatory Assay

scholarly journals Serological diagnosis of Chagas' disease: a potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi.

1997 ◽  
Vol 35 (7) ◽  
pp. 1829-1834 ◽  
Author(s):  
R P Mendes ◽  
S Hoshino-Shimizu ◽  
A M Moura da Silva ◽  
I Mota ◽  
R A Heredia ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Serological Diagnosis ◽  
Protein Antigens ◽  
Confirmatory Assay

scholarly journals Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera

1989 ◽  
Vol 84 (3) ◽  
pp. 309-314 ◽  
Author(s):  
M. G. Morgado ◽  
J. Ivo-dos-Santos ◽  
R. T. Pinho ◽  
E. Argüelles ◽  
J. M. Rezende ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Chagas Disease ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
The Other ◽  
Molecular Weights ◽  
Human Visceral Leishmaniasis ◽  
Human Immune

Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.

scholarly journals Simultaneous identification of Trypanosoma cruzi surface and internal antigens reactive to different immunoglobulin classes (radio-immunoblotting)

1990 ◽  
Vol 32 (5) ◽  
pp. 379-383 ◽  
Author(s):  
Anna Maria Simonsen Stolf ◽  
Eufrosina Setsu Umezawa ◽  
Bianca Zingales
Keyword(s):  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Chagas Disease ◽  
Western Blotting ◽  
Specific Antibodies ◽  
Internal Parasite ◽  
Sds Page ◽  
Blotting Technique ◽  
Simultaneous Identification ◽  
Acute Chagas Disease

A radioactive Western-blotting technique was developed by which the reactivity of Immunoglobulins (Igs) from different classes to both membrane radiolabelled and internal parasite antigens is simultaneously identified. The method includes radioiodination of parasites, polypeptide fractionation by SDS-PAGE, Western-blot transfer and autoradiography of the immunoblots developed with anti-Igs conjugates labelled with enzymes. The analysis is then performed by the comparison of common bands on the autoradiograms and the respective substrate stained nitrocellulose blots. This technique was used to analyse T. cruzi trypomastigote surface labelled antigens reactive to IgM, IgA and IgG specific antibodies. A different pattern of reactivity with acute Chagas' disease patients sera was thus obtained.

Chagas disease: recombinant Trypanosoma cruzi antigens for serological diagnosis

2001 ◽  
Vol 17 (6) ◽  
pp. 286-291 ◽  
Author(s):  
José Franco da Silveira ◽  
Eufrosina Setsu Umezawa ◽  
Alejandro Ostermayer Luquetti
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Serological Diagnosis

scholarly journals Use of two recombinant proteins of Trypanosoma cruzi in the serological diagnosis of Chagas disease

1990 ◽  
Vol 85 (4) ◽  
pp. 519-522 ◽  
Author(s):  
B. Zingales ◽  
A. Gruber ◽  
C. B. Ramalho ◽  
E. S. Umezawa ◽  
W. Colli
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Recombinant Proteins ◽  
Serological Diagnosis

scholarly journals Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1455
Author(s):  
Jean-Yves Brossas ◽  
Ballering Griselda ◽  
Margarita Bisio ◽  
Jeremy Guihenneuc ◽  
Julián Ernesto Nicolás Gulin ◽  
...  
Keyword(s):  
Western Blot ◽  
Chagas Disease ◽  
Predictive Value ◽  
Protozoan Parasite ◽  
World Health ◽  
Confirmatory Assay ◽  
Health Organization ◽  
Sensitivity Specificity ◽  
Initial Results ◽  
Cruzi Infection

Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.

PURIFIED TRYPANOSOMA CRUZI SPECIFIC GLYCOPROTEIN FOR DISCRIMINATIVE SEROLOGICAL DIAGNOSIS OF SOUTH AMERICAN TRYPANOSOMIASIS (CHAGAS' DISEASE)

The Lancet ◽  
1983 ◽  
Vol 322 (8356) ◽  
pp. 939-941 ◽  
Author(s):  
M Schechter ◽  
A Voller ◽  
C.J Marinkelle ◽  
J.E Flint ◽  
F Guhl ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Serological Diagnosis ◽  
South American ◽  
American Trypanosomiasis
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