Late allergic airway response to segmental bronchopulmonary provocation in allergic subjects is related to peripheral blood basophil histamine release

The aim of the study was to analyse the effect of interleukin-4 (IL-4) on allergen and anti-IgE mediated histamine release from basophils and human skin mast cells and to assess whether soluble recombinant interleukin-4 receptor (sIL4R) can inhibit these effects. Anti-IgE stimulated histamine release from peripheral blood basophils and mast cells of atopic donors was enhanced after preincubation with IL-4, whereas after preincubation with sIL-4R it was inhibited. These effects were even more pronounced when samples were stimulated with a clinically relevant allergen. In IL-4 preincubated skin mast cells, there was a similar enhancement of anti-IgE stimulated histamine release, which could again be inhibited by sIL-4R. The effects of IL-4 and sIL4R were dose- and time-dependent. Mice sensitized to ovalbumin and treated with soluble recombinant murine sIL-4R showed significantly reduced immediate-type cutaneous hypersensitivity responses compared with untreated mice. Thesein vivoeffects were IgE independent, since there were no significant differences in total and allergen specific IgE/IgG1 antibody titres between treated and untreated mice. This indicates that IL4 exerts priming effects on histamine release by effector cells of the allergic response and that these effects are potently antagonized by soluble IL-4R bothin vitroandin vivo.

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329 Interleukin-4 enhances and soluble interleukin-4 receptor inhibits histamine release from peripheral blood basophils and mast cells in vitro and in vivo

Journal of Allergy and Clinical Immunology ◽

10.1016/s0091-6749(96)80547-1 ◽

1996 ◽

Vol 97 (1) ◽

pp. 265-265

Author(s):

H RENZ ◽

B NIGGEMANN ◽

T ZUBERBIER ◽

U HERZ ◽

K ENSSLE ◽

...

Keyword(s):

Mast Cells ◽

Histamine Release ◽

Peripheral Blood ◽

Interleukin 4 ◽

Interleukin 4 Receptor

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Histamine release from peripheral blood leucocytes in egg-allergic patients

Clinical & Experimental Allergy ◽

10.1111/j.1365-2222.1986.tb01967.x ◽

1986 ◽

Vol 16 (4) ◽

pp. 345-354 ◽

Cited By ~ 10

Author(s):

P. M. CLINTON ◽

D. M. KEMENY ◽

P. AMLOT ◽

R. URBANEK ◽

M. H. LESSOF

Keyword(s):

Histamine Release ◽

Peripheral Blood ◽

Peripheral Blood Leucocytes

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Baseline Tryptase Levels Correlate with Baseline Basophil Levels in Chronic Myeloid Leukemia-Chronic Phase

Journal of Advances in Medicine and Medical Research ◽

10.9734/jammr/2020/v32i2330731 ◽

2020 ◽

pp. 171-176

Author(s):

Anisha Mathew ◽

Manisha Naithani ◽

Sarama Saha ◽

Rituparna Chetia ◽

Uttam Kumar Nath

Keyword(s):

Chronic Myeloid Leukemia ◽

Peripheral Blood ◽

Myeloid Leukemia ◽

Surrogate Marker ◽

Chronic Phase ◽

Newly Diagnosed ◽

Serum Tryptase ◽

Baseline Serum ◽

Tryptase Level ◽

Blood Basophil

Aims: To study whether there is any correlation between baseline blood basophil count and serum tryptase levels in newly diagnosed chronic phase chronic myeloid leukemia (CML-CP) patients. Settings and Design: 40 newly diagnosed CML-CP patients were enrolled from Medical Oncology Hematology OPD based on their baseline BCR-ABL status (done in department of Biochemistry). Methods and Materials: Serum tryptase level was measured using Sandwich ELISA and peripheral blood basophil count was estimated using automated cell counter & peripheral blood film examination. BCR-ABL quantification was done using real time PCR after conversion of RNA (extracted from whole blood) to cDNA. Statistical Analysis Used: SPSS Version 23. Results: Baseline peripheral blood basophil levels showed a significant correlation with baseline serum tryptase levels (p<0.01) and tryptase level also correlated with EUTOS score, which has basophil count as one of the parameters. This may signify that serum tryptase levels can be a surrogate marker of the basophil compartment in CML-CP. Conclusions: Based on findings of the present study and other studies available in literature, serum tryptase can be utilised as a surrogate marker of the basophil compartment in CML-CP.

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