Rapid detection of herpes simplex- and varicella-zoster virus antigens from clinical specimens by enzyme immunoassay

1985 ◽  
Vol 5 ◽  
pp. 107-110 ◽  
Author(s):  
Thedi Ziegler ◽  
Pekka E. Halonen
Keyword(s):  
Herpes Simplex ◽  
Varicella Zoster Virus ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Clinical Specimens ◽  
Varicella Zoster ◽  
Virus Antigens

scholarly journals Development and Validation of a Laboratory-Developed Multiplex Real-Time PCR Assay on the BD Max System for Detection of Herpes Simplex Virus and Varicella-Zoster Virus DNA in Various Clinical Specimens

2015 ◽  
Vol 53 (6) ◽  
pp. 1921-1926 ◽  
Author(s):  
Sylvie Pillet ◽  
Paul O. Verhoeven ◽  
Amélie Epercieux ◽  
Thomas Bourlet ◽  
Bruno Pozzetto
Keyword(s):  
Quality Control ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Varicella Zoster Virus ◽  
Internal Control ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Varicella Zoster ◽  
Simplex Virus

A multiplex real-time PCR (quantitative PCR [qPCR]) assay detecting herpes simplex virus (HSV) and varicella-zoster virus (VZV) DNA together with an internal control was developed on the BD Max platform combining automated DNA extraction and an open amplification procedure. Its performance was compared to those of PCR assays routinely used in the laboratory, namely, a laboratory-developed test for HSV DNA on the LightCycler instrument and a test using a commercial master mix for VZV DNA on the ABI7500fast system. Using a pool of negative cerebrospinal fluid (CSF) samples spiked with either calibrated controls for HSV-1 and VZV or dilutions of a clinical strain that was previously quantified for HSV-2, the empirical limit of detection of the BD Max assay was 195.65, 91.80, and 414.07 copies/ml for HSV-1, HSV-2, and VZV, respectively. All the samples from HSV and VZV DNA quality control panels (Quality Control for Molecular Diagnostics [QCMD], 2013, Glasgow, United Kingdom) were correctly identified by the BD Max assay. From 180 clinical specimens of various origins, 2 CSF samples were found invalid by the BD Max assay due to the absence of detection of the internal control; a concordance of 100% was observed between the BD Max assay and the corresponding routine tests. The BD Max assay detected the PCR signal 3 to 4 cycles earlier than did the routine methods. With results available within 2 h on a wide range of specimens, this sensitive and fully automated PCR assay exhibited the qualities required for detecting simultaneously HSV and VZV DNA on a routine basis.

Rapid detection of varicella-zoster virus in clinical specimens using monoclonal antibodies on shell vials and smears

1989 ◽  
Vol 28 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Janneke J. M. Meulenberg ◽  
Jurjen Schirm ◽  
Geke W. Pastoor ◽  
F. Peter Schröder ◽  
Pieter C. Voorst Van Vader
Keyword(s):  
Monoclonal Antibodies ◽  
Varicella Zoster Virus ◽  
Rapid Detection ◽  
Clinical Specimens ◽  
Varicella Zoster
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