Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein

2000 ◽  
Vol 76 (2-3) ◽  
pp. 215-226 ◽  
Author(s):  
P Jonasson
Keyword(s):  
Fusion Protein ◽  
Heat Release ◽  
C Peptide ◽  
Human Proinsulin ◽  
Integrated Bioprocess

scholarly journals Infrared Absorption Study of Human Proinsulin C-Peptide Fragments in Dichloromethane

1986 ◽  
Vol 59 (8) ◽  
pp. 2445-2449 ◽  
Author(s):  
Mitsuaki Narita ◽  
Toshihiko Ogura ◽  
Kazuhiro Sato ◽  
Shinya Honda
Keyword(s):  
Infrared Absorption ◽  
Absorption Study ◽  
Peptide Fragments ◽  
C Peptide ◽  
Human Proinsulin

A Monoclonal Antibody Applicable for Determination of C-Peptide of Human Proinsulin by RIA

Hybridoma ◽  
1991 ◽  
Vol 10 (3) ◽  
pp. 379-386 ◽  
Author(s):  
I. HILGERT ◽  
P. ŠTOLBA ◽  
H. KRIŠTOFOVÁ ◽  
I. ŠTEFANOV ◽  
B. BENDLOVÁ ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
C Peptide ◽  
Human Proinsulin

Studies on Polypeptides, VII. Synthesis, Circular Dichroism and Immunological Studien of Tyrosyl C-Peptide of Human Proinsulin

1975 ◽  
Vol 356 (2) ◽  
pp. 1305-1312 ◽  
Author(s):  
Vinod K. Naithani ◽  
Mathias Dechesne ◽  
Jan Markussen ◽  
Lise G. Heding ◽  
Ulla D. Larsen
Keyword(s):  
Circular Dichroism ◽  
C Peptide ◽  
Human Proinsulin ◽  
Circular Dichroïsm

Amplified Enzyme-Linked Immunoassay of Human Proinsulin in Serum (Detection Limit: 0.1 pmol/L)

1992 ◽  
Vol 38 (2) ◽  
pp. 227-232 ◽  
Author(s):  
F J Dhahir ◽  
D B Cook ◽  
C H Self
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Cross Reactivity ◽  
C Peptide ◽  
Human Proinsulin ◽  
Microtiter Plates ◽  
Enzyme Amplification ◽  
Fold Excess ◽  
Amplification Procedure ◽  
Serum Detection

Abstract We describe an amplified enzyme-linked immunoassay of human proinsulin in serum that detects intact proinsulin and both the 32/33 and 65/66 split forms. The method uses the IgG fraction of a polyclonal antibody raised in a guinea pig against intact proinsulin, which we used to coat plastic microtiter plates. A sandwich was formed with proinsulin by using a monoclonal antibody against C-peptide labeled with alkaline phosphatase. We quantified the reaction by using the enzyme amplification procedure, which detected as little intact proinsulin as 0.1 pmol/L. We found no cross-reactivity with C-peptide in the assay, and decreased recovery attributable to the presence of insulin could be demonstrated only with a 30-fold excess of this hormone over proinsulin.

Production of Antisera to Synthetic Benzyloxycarbonyl-C-peptide of Human Proinsulin

1976 ◽  
Vol 357 (1) ◽  
pp. 751-758 ◽  
Author(s):  
Ole K. FABER ◽  
Jan MARKUSSEN ◽  
Vinod K. NAITHANI ◽  
Christian BINDER
Keyword(s):  
C Peptide ◽  
Human Proinsulin

scholarly journals Radioimmunoassay of Human Proinsulin C-peptide Using Synthetic Human Connecting Peptide

1974 ◽  
Vol 21 (2) ◽  
pp. 141-145 ◽  
Author(s):  
TOSHIO KANEKO ◽  
HIROSHI OKA ◽  
MASAHIDE MUNEMURA ◽  
TOSHITSUGU ODA ◽  
KAMEJIRO YAMASHITA ◽  
...  
Keyword(s):  
C Peptide ◽  
Human Proinsulin

scholarly journals Studies on Human Proinsulin C-peptide Radioimmunoassay Method

RADIOISOTOPES ◽  
1977 ◽  
Vol 26 (5) ◽  
pp. 291-295
Author(s):  
Nobuhiko NAKAZAWA ◽  
Kentaro MAKI ◽  
Hiroshi OGAWA ◽  
Osamu IKEDA ◽  
Masanori SAKAGAMI ◽  
...  
Keyword(s):  
C Peptide ◽  
Human Proinsulin ◽  
Radioimmunoassay Method
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