A Monoclonal Antibody Applicable for Determination of C-Peptide of Human Proinsulin by RIA

Abstract We describe an amplified enzyme-linked immunoassay of human proinsulin in serum that detects intact proinsulin and both the 32/33 and 65/66 split forms. The method uses the IgG fraction of a polyclonal antibody raised in a guinea pig against intact proinsulin, which we used to coat plastic microtiter plates. A sandwich was formed with proinsulin by using a monoclonal antibody against C-peptide labeled with alkaline phosphatase. We quantified the reaction by using the enzyme amplification procedure, which detected as little intact proinsulin as 0.1 pmol/L. We found no cross-reactivity with C-peptide in the assay, and decreased recovery attributable to the presence of insulin could be demonstrated only with a 30-fold excess of this hormone over proinsulin.

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Evaluation of Three Commercially Available C-Peptide Kits

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328201900509 ◽

1982 ◽

Vol 19 (5) ◽

pp. 368-373 ◽

Cited By ~ 7

Author(s):

HJM Van Rijn ◽

JBL Hoekstra ◽

JHH Thijssen

Keyword(s):

Human Serum ◽

Cross Reactivity ◽

C Peptide ◽

Human Proinsulin ◽

Analytical Recovery

Three commercially available methods for the determination of C-peptide in human serum were evaluated. All kits were found to be acceptable with respect to precision and analytical recovery, while differences were observed with respect to sensitivity and cross-reactivity to human proinsulin. Furthermore an intermethod comparison was performed. It is pointed out that, before C-peptide determination on serum from insulin-treated diabetic subjects, a PEG precipitation, to remove antibody-bound proinsulin, should be done.

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PP001-MON DETERMINATION OF FRACTIONAL URINARY C-PEPTIDE TO ASSESS THE INSULIN SECRETION IN RESPONSE TO DIFFERENT TYPES OF ENTERAL NUTRITION FORMULA

Clinical Nutrition Supplements ◽

10.1016/s1744-1161(11)70293-9 ◽

2011 ◽

Vol 6 (1) ◽

pp. 115

Author(s):

A. Sanz-Paris ◽

A. Rodriguez-Valle ◽

D. Boj ◽

J.L. Gascon ◽

T. Corrales ◽

...

Keyword(s):

Insulin Secretion ◽

Enteral Nutrition ◽

C Peptide ◽

Different Types

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Improvement of a Monoclonal Antibody-based Immunoassay for the Determination of Terbutryn Verbesserung eines Immunassays mit monoklonalen Antikörpern zur Bestimmung von Terbutryn

Acta hydrochimica et hydrobiologica ◽

10.1002/aheh.19930210604 ◽

1993 ◽

Vol 21 (6) ◽

pp. 312-315 ◽

Cited By ~ 11

Author(s):

T. Giersch ◽

K. Kramer ◽

M. G. Weller ◽

B. Hock

Keyword(s):

Monoclonal Antibody

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Determination of 1,5‒anhydro‒glucitol–carrier protein conjugates by matrix‒assisted laser desorption/ionization tof mass spectrometry and antibody formation

Spectroscopy An International Journal ◽

10.1155/2000/151704 ◽

2000 ◽

Vol 14 (3) ◽

pp. 85-92 ◽

Cited By ~ 8

Author(s):

Li-Jiang Xuan ◽

Hiroyuki Tanaka ◽

Satoshi Morimoto ◽

Yukihiro Shoyama ◽

Hiroshi Akanuma ◽

...

Keyword(s):

Monoclonal Antibody ◽

Serum Albumin ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Protein Conjugates ◽

Desorption Ionization ◽

Chicken Lysozyme ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

In order to prepare the monoclonal antibody against 1,5-anhydro-glucitol (1), it was conjugated with bovine serum albumin (BSA), human serum albumin (HSA) or chicken lysozyme (CL) using succinate orβ-alanine succinate as a spacer to produce individual antigen conjugates. The number of hapten contained in each antigen conjugate was determined by matrix-assisted laser desorption/ionization tof mass (MALDI-tof-MS) spectrometry. The formation of monoclonal antibody (MAb) was also discussed.

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