The thermal stability of RNA duplexes containing modified base pairs placed at internal and terminal positions of the oligoribonucleotides

2002 ◽  
Vol 97 (2-3) ◽  
pp. 233-241 ◽  
Author(s):  
Krzysztof Ziomek ◽  
Elżbieta Kierzek ◽  
Ewa Biała ◽  
Ryszard Kierzek
Keyword(s):  
Thermal Stability ◽  
Base Pairs ◽  
Rna Duplexes ◽  
Thermal Stability Of

The influence of various modified nucleotides placed as 3′-dangling end on thermal stability of RNA duplexes

2002 ◽  
Vol 97 (2-3) ◽  
pp. 243-249 ◽  
Author(s):  
Krzysztof Ziomek ◽  
Elżbieta Kierzek ◽  
Ewa Biała ◽  
Ryszard Kierzek
Keyword(s):  
Thermal Stability ◽  
Modified Nucleotides ◽  
Rna Duplexes ◽  
Thermal Stability Of

scholarly journals Second generation silver(I)-mediated imidazole base pairs

2014 ◽  
Vol 10 ◽  
pp. 2139-2144 ◽  
Author(s):  
Susanne Hensel ◽  
Nicole Megger ◽  
Kristina Schweizer ◽  
Jens Müller
Keyword(s):  
Thermal Stability ◽  
Melting Temperature ◽  
Base Pair ◽  
Metal Ion ◽  
Second Generation ◽  
Dna Duplex ◽  
Base Pairs ◽  
Double Helices ◽  
Thermal Stability Of

The imidazole–Ag(I)–imidazole base pair is one of the best-investigated artificial metal-mediated base pairs. We show here that its stability can be further improved by formally replacing the imidazole moiety by a 2-methylimidazole or 4-methylimidazole moiety. A comparison of the thermal stability of several double helices shows that the addition of one equivalent of Ag(I) leads to a 50% larger increase in the melting temperature when a DNA duplex with methylated imidazole nucleosides is applied. This significant effect can likely be attributed to a better steric shielding of the metal ion within the metal-mediated base pair.

scholarly journals 4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes

2021 ◽  
pp. 100231
Author(s):  
Ryo Tsukimura ◽  
Ryohei Kajino ◽  
Yujun Zhou ◽  
Akash Chandela ◽  
Yoshihito Ueno
Keyword(s):  
Thermal Stability ◽  
Rna Duplexes ◽  
Nuclease Resistance ◽  
Thermal Stability Of
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