Supersensitization of the adenylyl cyclase system in Chinese hamster ovary cells co-expressing cloned opioid receptors and Gz, a PTX-insensitive G protein

1999 ◽  
Vol 267 (2) ◽  
pp. 117-120 ◽  
Author(s):  
Tohru Ozawa ◽  
Takayuki Nakagawa ◽  
Masabumi Minami ◽  
Masamichi Satoh
Keyword(s):  
Adenylyl Cyclase ◽  
G Protein ◽  
Opioid Receptors ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Adenylyl Cyclase System

Partial Agonistic Activity of Naloxone on the Opioid Receptors Expressed from Complementary Deoxyribonucleic Acids in Chinese Hamster Ovary Cells

1998 ◽  
Vol 87 (2) ◽  
pp. 450-455 ◽  
Author(s):  
Kazuhiko Fukuda ◽  
Shigehisa Kato ◽  
Takehiro Shoda ◽  
Hitoshi Morikawa ◽  
Hiroyuki Mima ◽  
...  
Keyword(s):  
Opioid Receptors ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Agonistic Activity

Interaction of Local Anesthetics with Recombinant Opioid Receptors Expressed in Chinese Hamster Ovary Cells

1998 ◽  
Vol 89 (Supplement) ◽  
pp. 733A
Author(s):  
Kazuyoshi Hirota ◽  
Balraj L Appadu ◽  
David K Grandy ◽  
Lakshmi A Devi ◽  
David G Lambert
Keyword(s):  
Opioid Receptors ◽  
Chinese Hamster Ovary ◽  
Local Anesthetics ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Effect of Lidocaine on cAMP Formation with Recombinant Opioid Receptors Expressed in Chinese Hamster Ovary Cells

1998 ◽  
Vol 89 (Supplement) ◽  
pp. 802A ◽  
Author(s):  
Kazuyoshi Hirota ◽  
Balraj L Appadu ◽  
David K Grandy ◽  
Lakshmi A Devi ◽  
David G Lambert
Keyword(s):  
Opioid Receptors ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Camp Formation

scholarly journals A Real-Time, Fluorescence-Based Assay for Measuring µ-Opioid Receptor Modulation of Adenylyl Cyclase Activity in Chinese Hamster Ovary Cells

2013 ◽  
Vol 19 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Alisa Knapman ◽  
Fe Abogadie ◽  
Peter McIntrye ◽  
Mark Connor
Keyword(s):  
Adenylyl Cyclase ◽  
Real Time ◽  
Opioid Receptor ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Dependent Manner ◽  
Simultaneous Application ◽  
Ovary Cells ◽  
Receptor Modulation

Inhibition of adenylyl cyclase (AC) activity is frequently used to measure µ-opioid receptor (MOR) activation. We sought to develop a simple, rapid assay of AC activity in whole cells that could be used to study MOR signaling. Chinese hamster ovary cells expressing human MOR (CHO-MOR cells) were grown in 96-well plates and loaded with membrane potential–sensitive fluorescent dye. CHO-MOR cells were treated with the AC activator forskolin (FSK), with or without simultaneous application of MOR agonists, and the resulting change in fluorescence was measured. CHO-MOR cells hyperpolarized in response to application of FSK ( pEC50, 7.3) or calcitonin ( pEC50, 9.4). A submaximally effective concentration of FSK (300 nM) caused a 52% ± 2% decrease in fluorescence. Simultaneous application of the opioids DAMGO ( pEC50, 7.4; Emax, 56%), morphine ( pEC50, 7.0; Emax, 61%); and buprenorphine ( pEC50, 8.6; Emax, 24%) inhibited the FSK response in a dose-dependent manner while having no effect by themselves. The effects of DAMGO were blocked by pertussis toxin. This assay represents a simple, robust method for real-time observation of AC inhibition by MOR in CHO cells. It represents an appealing alternative to end-point assays that rely on cAMP accumulation and can avoid potential confounds associated with rapid desensitization of MOR signaling.

Rhodopsin expressed in Chinese hamster ovary cells regulates adenylyl cyclase activity

1990 ◽  
Vol 4 (1) ◽  
pp. 71-79 ◽  
Author(s):  
E. R. Weiss ◽  
R. A. Heller-Harrison ◽  
E. Diez ◽  
M. Crasnier ◽  
C. C. Malbon ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cyclase Activity ◽  
Adenylyl Cyclase Activity ◽  
Ovary Cells
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