Photosensitized continuous production of hydrogen by Halobacterium halobium MMT22 coupled to Escherichia coli

1997 ◽  
Vol 22 (10-11) ◽  
pp. 995-997 ◽  
Author(s):  
M KHAN
Keyword(s):  
Escherichia Coli ◽  
Continuous Production ◽  
Halobacterium Halobium ◽  
Production Of Hydrogen

scholarly journals Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant

2017 ◽  
Vol 30 ◽  
pp. 64-70 ◽  
Author(s):  
Chiu-Shyan Soo ◽  
Wai-Sum Yap ◽  
Wei-Min Hon ◽  
Norhayati Ramli ◽  
Umi Kalsom Md Shah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Production Of Hydrogen

Continuous production of d-lactic acid from cellobiose in cell recycle fermentation using β-glucosidase-displaying Escherichia coli

2019 ◽  
Vol 127 (4) ◽  
pp. 441-446 ◽  
Author(s):  
Yuji Aso ◽  
Mikikazu Tsubaki ◽  
Bui Hoang Dang Long ◽  
Ryo Murakami ◽  
Keisuke Nagata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Continuous Production ◽  
Cell Recycle

scholarly journals Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases.

2021 ◽  
Author(s):  
Magali Roger ◽  
Thomas C. P. Reed ◽  
Frank Sargent
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Formic Acid ◽  
Carbon Capture ◽  
Continuous Production ◽  
Carbon Capture And Storage ◽  
Physiological Role ◽  
Anaerobic Growth ◽  
Formate Hydrogenlyase ◽  
And Storage

Escherichia coli is gram-negative bacterium that is a workhorse for biotechnology. The organism naturally performs a mixed-acid fermentation under anaerobic conditions where it synthesises formate hydrogenlyase (FHL-1). The physiological role of the enzyme is the disproportionation of formate in to H 2 and CO 2 . However, the enzyme has been observed to catalyse hydrogenation of CO 2 given the correct conditions, and so has possibilities in bio-based carbon capture and storage if it can be harnessed as a hydrogen-dependent CO 2 -reductase (HDCR). In this study, an E. coli host strain was engineered for the continuous production of formic acid from H 2 and CO 2 during bacterial growth in a pressurised batch bioreactor. Incorporation of tungsten, in place of molybdenum, in FHL-1 helped to impose a degree of catalytic bias on the enzyme. This work demonstrates that it is possible to couple cell growth to simultaneous, unidirectional formate production from carbon dioxide and develops a process for growth under pressurised gases. IMPORTANCE Greenhouse gas emissions, including waste carbon dioxide, are contributing to global climate change. A basket of solutions is needed to steadily reduce emissions, and one approach is bio-based carbon capture and storage. Here we present out latest work on harnessing a novel biological solution for carbon capture. The Escherichia coli formate hydrogenlyase (FHL-1) was engineered to be constitutively expressed. Anaerobic growth under pressurised H 2 and CO 2 gases was established and aqueous formic acid was produced as a result. Incorporation of tungsten in to the enzyme in place of molybdenum proved useful in poising FHL-1 as a hydrogen-dependent CO 2 reductase (HDCR).

Application of Escherichia coli maltodextrin-phosphorylase for the continuous production of glucose-1-phosphate

1995 ◽  
Vol 17 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Andreas Weinhäusel ◽  
Bernd Nidetzky ◽  
Christian Kysela ◽  
Klaus D. Kulbe
Keyword(s):  
Escherichia Coli ◽  
Continuous Production ◽  
Maltodextrin Phosphorylase
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