Continuous production of recombinant interferon-α in Escherichia coli via the derepression of trp promoter using casamino acid

2006 ◽  
Vol 41 (4) ◽  
pp. 809-814 ◽  
Author(s):  
Kyung-Hwan Jung
Keyword(s):  
Escherichia Coli ◽  
Continuous Production ◽  
Casamino Acid ◽  
Interferon Α ◽  
Recombinant Interferon ◽  
Trp Promoter

Improvement of soluble recombinant interferon-α expression by methyl α-D-glucopyranoside in araBAD promoter system of Escherichia coli

2009 ◽  
Vol 14 (3) ◽  
pp. 274-280 ◽  
Author(s):  
Kyung-Hwan Jung ◽  
You-Jin Lee ◽  
Ji-Hyeon Yeon ◽  
Sun Kyun Yoo ◽  
Byeong-Churl Chung
Keyword(s):  
Escherichia Coli ◽  
Interferon Α ◽  
Recombinant Interferon ◽  
Promoter System

Allergy of delayed type to recombinant interferon α 2c

1989 ◽  
Vol 20 (2) ◽  
pp. 149-150 ◽  
Author(s):  
Ulf Detmar ◽  
Monika Agathos ◽  
Christoph Nerl
Keyword(s):  
Interferon Α ◽  
Recombinant Interferon

Continuous production of d-lactic acid from cellobiose in cell recycle fermentation using β-glucosidase-displaying Escherichia coli

2019 ◽  
Vol 127 (4) ◽  
pp. 441-446 ◽  
Author(s):  
Yuji Aso ◽  
Mikikazu Tsubaki ◽  
Bui Hoang Dang Long ◽  
Ryo Murakami ◽  
Keisuke Nagata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Continuous Production ◽  
Cell Recycle

scholarly journals Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases.

2021 ◽  
Author(s):  
Magali Roger ◽  
Thomas C. P. Reed ◽  
Frank Sargent
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
Formic Acid ◽  
Carbon Capture ◽  
Continuous Production ◽  
Carbon Capture And Storage ◽  
Physiological Role ◽  
Anaerobic Growth ◽  
Formate Hydrogenlyase ◽  
And Storage

Escherichia coli is gram-negative bacterium that is a workhorse for biotechnology. The organism naturally performs a mixed-acid fermentation under anaerobic conditions where it synthesises formate hydrogenlyase (FHL-1). The physiological role of the enzyme is the disproportionation of formate in to H 2 and CO 2 . However, the enzyme has been observed to catalyse hydrogenation of CO 2 given the correct conditions, and so has possibilities in bio-based carbon capture and storage if it can be harnessed as a hydrogen-dependent CO 2 -reductase (HDCR). In this study, an E. coli host strain was engineered for the continuous production of formic acid from H 2 and CO 2 during bacterial growth in a pressurised batch bioreactor. Incorporation of tungsten, in place of molybdenum, in FHL-1 helped to impose a degree of catalytic bias on the enzyme. This work demonstrates that it is possible to couple cell growth to simultaneous, unidirectional formate production from carbon dioxide and develops a process for growth under pressurised gases. IMPORTANCE Greenhouse gas emissions, including waste carbon dioxide, are contributing to global climate change. A basket of solutions is needed to steadily reduce emissions, and one approach is bio-based carbon capture and storage. Here we present out latest work on harnessing a novel biological solution for carbon capture. The Escherichia coli formate hydrogenlyase (FHL-1) was engineered to be constitutively expressed. Anaerobic growth under pressurised H 2 and CO 2 gases was established and aqueous formic acid was produced as a result. Incorporation of tungsten in to the enzyme in place of molybdenum proved useful in poising FHL-1 as a hydrogen-dependent CO 2 reductase (HDCR).

scholarly journals Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.

1983 ◽  
Vol 153 (2) ◽  
pp. 1120-1123 ◽  
Author(s):  
G Bogosian ◽  
P V Haydock ◽  
R L Somerville
Keyword(s):  
Escherichia Coli ◽  
Trp Promoter ◽  
Stimulation Of
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