Rapid identification of the species of theBacteroides fragilisgroup by multiplex PCR assays using group- and species-specific primers

ABSTRACT Bifidobacteria are one of the most common bacterial types found in the intestines of humans and other animals and may be used as indicators of human fecal pollution. The presence of nine human-related Bifidobacterium species was analyzed in human and animal wastewater samples of different origins by using species-specific primers based on 16S rRNA sequences. Only B. adolescentis and B. dentium were found exclusively in human sewage. A multiplex PCR approach with strain-specific primers was developed. The method showed a sensitivity threshold of 10 cells/ml. This new molecular method could provide useful information for the characterization of fecal pollution sources.

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Rapid and Specific Identification of Genus Cynoglossus by Multiplex PCR Assays Using Species-specific Derived from the COI Region

Journal of Life Science ◽

10.5352/jls.2016.26.9.1007 ◽

2016 ◽

Vol 26 (9) ◽

pp. 1007-1014 ◽

Cited By ~ 1

Author(s):

Eun Soo Noh ◽

Hyun Sook Kang ◽

Cheul Min An ◽

Jung Youn Park ◽

Eun Mi Kim ◽

...

Keyword(s):

Multiplex Pcr ◽

Specific Identification ◽

Pcr Assays ◽

Species Specific

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Development of species‐specific primers for rapid identification by PCR of the ecologically important pathogen Fusarium keratoplasticum from isolated and environmental samples

Letters in Applied Microbiology ◽

10.1111/lam.13545 ◽

2021 ◽

Author(s):

Alexandra F. Agee ◽

Michelle M. Barthet

Keyword(s):

Environmental Samples ◽

Rapid Identification ◽

Specific Primers ◽

Species Specific

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Rapid identification of the species of theBacteroides fragilisgroup by multiplex PCR assays using group- and species-specific primers

Rapid identification of 11 human intestinalLactobacillusspecies by multiplex PCR assays using group- and species-specific primers derived from the 16Sâ23S rRNA intergenic spacer region and its flanking 23S rRNA

Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S–23S rRNA intergenic spacer region and its flanking 23S rRNA

Rapid identification of probioticLactobacillusspecies by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA

Rapid identification of Saccharomonospora strains by multiplex PCR using species-specific primers within the 16S rRNA gene

Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilus group using multiplex PCR

Rapid identification of potentially probioticBifidobacteriumspecies by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA

Application of multiplex PCR using species-specific primers within the 16S rRNA gene for rapid identification of Nocardioides strains

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

Rapid and Specific Identification of Genus Cynoglossus by Multiplex PCR Assays Using Species-specific Derived from the COI Region

Development of species‐specific primers for rapid identification by PCR of the ecologically important pathogen Fusarium keratoplasticum from isolated and environmental samples

Rapid identification of 11 human intestinalLactobacillusspecies by multiplex PCR assays using group- and species-specific primers derived from the 16Sâ23S rRNA intergenic spacer region and its flanking 23S rRNA