Molecular analysis of the Rhodobacter capsulatus chaperone dnaKJ operon: purification and characterization of DnaK

Gene ◽  
1997 ◽  
Vol 192 (2) ◽  
pp. 251-259 ◽  
Author(s):  
Christiane M Nickel ◽  
Joel Vandekerckhove ◽  
Peter Beyer ◽  
Monier H Tadros
1996 ◽  
Vol 166 (3) ◽  
pp. 193-203 ◽  
Author(s):  
Philipp Hübner ◽  
Gregory Dame ◽  
Ursula Sandmeier ◽  
Joel Vandekerckhove ◽  
Peter Beyer ◽  
...  

1993 ◽  
Vol 268 (14) ◽  
pp. 10636-10644
Author(s):  
Y. Jouanneau ◽  
C. Meyer ◽  
J. Gaillard ◽  
E. Forest ◽  
J. Gagnon

1990 ◽  
Vol 171 (1) ◽  
pp. 273-279 ◽  
Author(s):  
Yves Jouanneau ◽  
Christine Meyer ◽  
Jacques Gaillard ◽  
Paulette M. Vignais

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.


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