Repair of 8-oxoguanine in Saccharomyces cerevisiae: interplay of DNA repair and replication mechanisms2,3 2Guest Editor: Miral Dizdaroglu 3This article is part of a series of reviews on “Oxidative DNA Damage and Repair.” The full list of papers may be found on the homepage of the journal.

2002 ◽  
Vol 32 (12) ◽  
pp. 1244-1253 ◽  
Author(s):  
Serge Boiteux ◽  
Lionel Gellon ◽  
Nathalie Guibourt
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Damage ◽  
Dna Repair ◽  
Oxidative Dna Damage ◽  
Dna Damage And Repair ◽  
Full List

scholarly journals DNA damage and repair in a model of rat vascular injury

2010 ◽  
Vol 118 (7) ◽  
pp. 473-485 ◽  
Author(s):  
Amalia Forte ◽  
Mauro Finicelli ◽  
Mario Grossi ◽  
Mariano Vicchio ◽  
Nicola Alessio ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Oxidative Dna Damage ◽  
Vascular Wall ◽  
Tissue Expression ◽  
Dna Damage And Repair ◽  
Reverse Transcription Pcr ◽  
Relevant Role ◽  
Temporary Decrease ◽  
Damage Marker

Restenosis rates following vascular interventions still limit their long-term success. Oxidative stress plays a relevant role in this pathophysiological phenomenon, but less attention has been devoted to its effects on DNA damage and to the subsequent mechanisms of repair. In the present study, we analysed in a model of arteriotomy-induced stenosis in rat carotid arteries the time-dependent expression of DNA damage markers and of DNA repair genes, together with the assessment of proliferation and apoptosis indexes. The expression of the oxidative DNA damage marker 7,8-dihydro-8-oxo-2′-deoxyguanosine was increased at 3 and 7 days after arteriotomy, with immunostaining distributed in the injured vascular wall and perivascular tissue. Expression of the DNA damage marker phospho-H2A.X was less relevant, but increased from 4 h to 7 days after arteriotomy, with immunostaining prevalently present in the adventitia and, to a lesser extent, in medial smooth muscle cells at the injury site. RT (reverse transcription)–PCR indicated a decrease in eight out of 12 genes involved in the DNA repair machinery we selected from 4 h to 7 days after arteriotomy, with the exception of an increase in the Mutyh and Slk genes (P<0.05). Western blot analysis revealed a decrease in p53 and catalase at 3 days after arteriotomy (P<0.05). A maximal 7% of BrdU-positive cells in the endothelium and media occurred at 7 days after arteriotomy, whereas the apoptotic index peaked at 3 days after injury (P<0.05). In conclusion, our results highlight a persistent DNA damage, presumably related to a temporary decrease in the expression of the DNA repair machinery and of the antioxidant enzyme catalase, playing a role in stenosis progression.

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